Myricetin, a naturally occurring flavonoid, prevents 2-deoxy-d-ribose induced dysfunction and oxidative damage in osteoblastic MC3T3-E1 cells

Kyung Hee Lee; Eun Mi Choi

doi:10.1016/j.ejphar.2008.06.004

Myricetin, a naturally occurring flavonoid, prevents 2-deoxy-d-ribose induced dysfunction and oxidative damage in osteoblastic MC3T3-E1 cells

Kyung Hee Lee
, Eun Mi Choi

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Myricetin, a naturally occurring flavonoid, was investigated to determine whether it could protect osteoblasts from 2-deoxy-d-ribose induced dysfunction and oxidative damage in the MC3T3-E1 cells. MC3T3-E1 cells were incubated with 2-deoxy-d-ribose and/or myricetin, and markers of osteoblast function and oxidative damage were examined. Compared with control incubation, 2-deoxy-d-ribose significantly (P < 0.05) inhibited alkaline phosphatase (ALP) activity, collagen content, and calcium deposition at the concentration of 20 mM. Cellular malondialdehyde (MDA), protein carbonyl, and advanced oxidation protein products contents were significantly (P < 0.05) increased in the presence of 2-deoxy-d-ribose (20 mM). Myricetin significantly (P < 0.05) increased cell survival, ALP activity, collagen, osteocalcin, osteoprotegerin, and calcium deposition and decreased MDA, protein carbonyl, and advanced oxidation protein products contents of osteoblastic MC3T3-E1 cells in the presence of 20 mM 2-deoxy-d-ribose. These results demonstrate that myricetin attenuates 2-deoxy-d-ribose induced damage, suggesting that myricetin may be a useful dietary supplement for minimizing oxidative injury in diabetes related bone diseases.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	European Journal of Pharmacology
Volume	591
Issue number	1-3
DOIs	https://doi.org/10.1016/j.ejphar.2008.06.004
Publication status	Published - 4 Sept 2008

Bibliographical note

Funding Information:
This work was supported by a grant of the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2005-005-J13002).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

2-Deoxy-d-ribose
Glucose toxicity
Myricetin
Oxidative stress

Access to Document

10.1016/j.ejphar.2008.06.004

Cite this

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title = "Myricetin, a naturally occurring flavonoid, prevents 2-deoxy-d-ribose induced dysfunction and oxidative damage in osteoblastic MC3T3-E1 cells",

abstract = "Myricetin, a naturally occurring flavonoid, was investigated to determine whether it could protect osteoblasts from 2-deoxy-d-ribose induced dysfunction and oxidative damage in the MC3T3-E1 cells. MC3T3-E1 cells were incubated with 2-deoxy-d-ribose and/or myricetin, and markers of osteoblast function and oxidative damage were examined. Compared with control incubation, 2-deoxy-d-ribose significantly (P < 0.05) inhibited alkaline phosphatase (ALP) activity, collagen content, and calcium deposition at the concentration of 20 mM. Cellular malondialdehyde (MDA), protein carbonyl, and advanced oxidation protein products contents were significantly (P < 0.05) increased in the presence of 2-deoxy-d-ribose (20 mM). Myricetin significantly (P < 0.05) increased cell survival, ALP activity, collagen, osteocalcin, osteoprotegerin, and calcium deposition and decreased MDA, protein carbonyl, and advanced oxidation protein products contents of osteoblastic MC3T3-E1 cells in the presence of 20 mM 2-deoxy-d-ribose. These results demonstrate that myricetin attenuates 2-deoxy-d-ribose induced damage, suggesting that myricetin may be a useful dietary supplement for minimizing oxidative injury in diabetes related bone diseases.",

keywords = "2-Deoxy-d-ribose, Glucose toxicity, Myricetin, Oxidative stress",

author = "Lee, \{Kyung Hee\} and Choi, \{Eun Mi\}",

note = "Funding Information: This work was supported by a grant of the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2005-005-J13002).",

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doi = "10.1016/j.ejphar.2008.06.004",

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AU - Lee, Kyung Hee

AU - Choi, Eun Mi

N1 - Funding Information: This work was supported by a grant of the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2005-005-J13002).

PY - 2008/9/4

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N2 - Myricetin, a naturally occurring flavonoid, was investigated to determine whether it could protect osteoblasts from 2-deoxy-d-ribose induced dysfunction and oxidative damage in the MC3T3-E1 cells. MC3T3-E1 cells were incubated with 2-deoxy-d-ribose and/or myricetin, and markers of osteoblast function and oxidative damage were examined. Compared with control incubation, 2-deoxy-d-ribose significantly (P < 0.05) inhibited alkaline phosphatase (ALP) activity, collagen content, and calcium deposition at the concentration of 20 mM. Cellular malondialdehyde (MDA), protein carbonyl, and advanced oxidation protein products contents were significantly (P < 0.05) increased in the presence of 2-deoxy-d-ribose (20 mM). Myricetin significantly (P < 0.05) increased cell survival, ALP activity, collagen, osteocalcin, osteoprotegerin, and calcium deposition and decreased MDA, protein carbonyl, and advanced oxidation protein products contents of osteoblastic MC3T3-E1 cells in the presence of 20 mM 2-deoxy-d-ribose. These results demonstrate that myricetin attenuates 2-deoxy-d-ribose induced damage, suggesting that myricetin may be a useful dietary supplement for minimizing oxidative injury in diabetes related bone diseases.

AB - Myricetin, a naturally occurring flavonoid, was investigated to determine whether it could protect osteoblasts from 2-deoxy-d-ribose induced dysfunction and oxidative damage in the MC3T3-E1 cells. MC3T3-E1 cells were incubated with 2-deoxy-d-ribose and/or myricetin, and markers of osteoblast function and oxidative damage were examined. Compared with control incubation, 2-deoxy-d-ribose significantly (P < 0.05) inhibited alkaline phosphatase (ALP) activity, collagen content, and calcium deposition at the concentration of 20 mM. Cellular malondialdehyde (MDA), protein carbonyl, and advanced oxidation protein products contents were significantly (P < 0.05) increased in the presence of 2-deoxy-d-ribose (20 mM). Myricetin significantly (P < 0.05) increased cell survival, ALP activity, collagen, osteocalcin, osteoprotegerin, and calcium deposition and decreased MDA, protein carbonyl, and advanced oxidation protein products contents of osteoblastic MC3T3-E1 cells in the presence of 20 mM 2-deoxy-d-ribose. These results demonstrate that myricetin attenuates 2-deoxy-d-ribose induced damage, suggesting that myricetin may be a useful dietary supplement for minimizing oxidative injury in diabetes related bone diseases.

KW - 2-Deoxy-d-ribose

KW - Glucose toxicity

KW - Myricetin

KW - Oxidative stress

UR - https://www.scopus.com/pages/publications/48749084360

U2 - 10.1016/j.ejphar.2008.06.004

DO - 10.1016/j.ejphar.2008.06.004

M3 - Article

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JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 1-3

ER -

Myricetin, a naturally occurring flavonoid, prevents 2-deoxy-d-ribose induced dysfunction and oxidative damage in osteoblastic MC3T3-E1 cells

Abstract

Bibliographical note

UN SDGs

Keywords

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