Neuronal Regulation of the Spatial Patterning of Neurogenesis

Rosa Gonzalez-Quevedo; Yoonsung Lee; Kenneth D. Poss; David G. Wilkinson

doi:10.1016/j.devcel.2009.11.010

Neuronal Regulation of the Spatial Patterning of Neurogenesis

Rosa Gonzalez-Quevedo
, Yoonsung Lee
, Kenneth D. Poss
, David G. Wilkinson

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

Precise regulation of neurogenesis is achieved in specific regions of the vertebrate nervous system by formation of distinct neurogenic and nonneurogenic zones. We have investigated how neurogenesis becomes confined to zones adjacent to rhombomere boundaries in the zebrafish hindbrain. The nonneurogenic zone at segment centers comprises a distinct progenitor population that expresses fibroblast growth factor (fgfr) 2, erm, sox9b, and the retinoic acid degrading enzyme, cyp26b1. FGF receptor activation upregulates expression of these genes and inhibits neurogenesis in segment centers. Cyp26 activity is a key effector inhibiting neuronal differentiation, suggesting antagonistic interactions with retinoid signaling. We identify the critical FGF ligand, fgf20a, which is expressed by specific neurons located in the mantle region at the center of segments, adjacent to the nonneurogenic zone. Fgf20a mutants have ectopic neurogenesis and lack the segment center progenitor population. Our findings reveal how signaling from neurons induces formation of a nonneurogenic zone of neural progenitors.

Original language	English
Pages (from-to)	136-147
Number of pages	12
Journal	Developmental Cell
Volume	18
Issue number	1
DOIs	https://doi.org/10.1016/j.devcel.2009.11.010
Publication status	Published - 19 Jan 2010

Bibliographical note

Funding Information:
We thank Sebastian Gerety, Francois Guillemot, James Briscoe, Qiling Xu, and Javier Terriente for advice and comments on the manuscript, Silvana Gioli for anti-Sox9 antibody, Cecilia Moens for cyp26b1 probe, Ivor Mason for fgfr2 probe, David Raible and Alex Nechiporuk for fgf genes, and Janssen Pharmaceutica for Cyp26 inhibitor. This work was supported by the Medical Research Council, a Marie Curie Intra-European Fellowship (R.G.-Q.), and the National Institutes of Health and Pew Charitable Trusts (K.D.P.).

Keywords

DEVBIO
MOLNEURO

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title = "Neuronal Regulation of the Spatial Patterning of Neurogenesis",

abstract = "Precise regulation of neurogenesis is achieved in specific regions of the vertebrate nervous system by formation of distinct neurogenic and nonneurogenic zones. We have investigated how neurogenesis becomes confined to zones adjacent to rhombomere boundaries in the zebrafish hindbrain. The nonneurogenic zone at segment centers comprises a distinct progenitor population that expresses fibroblast growth factor (fgfr) 2, erm, sox9b, and the retinoic acid degrading enzyme, cyp26b1. FGF receptor activation upregulates expression of these genes and inhibits neurogenesis in segment centers. Cyp26 activity is a key effector inhibiting neuronal differentiation, suggesting antagonistic interactions with retinoid signaling. We identify the critical FGF ligand, fgf20a, which is expressed by specific neurons located in the mantle region at the center of segments, adjacent to the nonneurogenic zone. Fgf20a mutants have ectopic neurogenesis and lack the segment center progenitor population. Our findings reveal how signaling from neurons induces formation of a nonneurogenic zone of neural progenitors.",

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author = "Rosa Gonzalez-Quevedo and Yoonsung Lee and Poss, \{Kenneth D.\} and Wilkinson, \{David G.\}",

note = "Funding Information: We thank Sebastian Gerety, Francois Guillemot, James Briscoe, Qiling Xu, and Javier Terriente for advice and comments on the manuscript, Silvana Gioli for anti-Sox9 antibody, Cecilia Moens for cyp26b1 probe, Ivor Mason for fgfr2 probe, David Raible and Alex Nechiporuk for fgf genes, and Janssen Pharmaceutica for Cyp26 inhibitor. This work was supported by the Medical Research Council, a Marie Curie Intra-European Fellowship (R.G.-Q.), and the National Institutes of Health and Pew Charitable Trusts (K.D.P.). ",

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AU - Wilkinson, David G.

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N2 - Precise regulation of neurogenesis is achieved in specific regions of the vertebrate nervous system by formation of distinct neurogenic and nonneurogenic zones. We have investigated how neurogenesis becomes confined to zones adjacent to rhombomere boundaries in the zebrafish hindbrain. The nonneurogenic zone at segment centers comprises a distinct progenitor population that expresses fibroblast growth factor (fgfr) 2, erm, sox9b, and the retinoic acid degrading enzyme, cyp26b1. FGF receptor activation upregulates expression of these genes and inhibits neurogenesis in segment centers. Cyp26 activity is a key effector inhibiting neuronal differentiation, suggesting antagonistic interactions with retinoid signaling. We identify the critical FGF ligand, fgf20a, which is expressed by specific neurons located in the mantle region at the center of segments, adjacent to the nonneurogenic zone. Fgf20a mutants have ectopic neurogenesis and lack the segment center progenitor population. Our findings reveal how signaling from neurons induces formation of a nonneurogenic zone of neural progenitors.

AB - Precise regulation of neurogenesis is achieved in specific regions of the vertebrate nervous system by formation of distinct neurogenic and nonneurogenic zones. We have investigated how neurogenesis becomes confined to zones adjacent to rhombomere boundaries in the zebrafish hindbrain. The nonneurogenic zone at segment centers comprises a distinct progenitor population that expresses fibroblast growth factor (fgfr) 2, erm, sox9b, and the retinoic acid degrading enzyme, cyp26b1. FGF receptor activation upregulates expression of these genes and inhibits neurogenesis in segment centers. Cyp26 activity is a key effector inhibiting neuronal differentiation, suggesting antagonistic interactions with retinoid signaling. We identify the critical FGF ligand, fgf20a, which is expressed by specific neurons located in the mantle region at the center of segments, adjacent to the nonneurogenic zone. Fgf20a mutants have ectopic neurogenesis and lack the segment center progenitor population. Our findings reveal how signaling from neurons induces formation of a nonneurogenic zone of neural progenitors.

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KW - MOLNEURO

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Neuronal Regulation of the Spatial Patterning of Neurogenesis

Abstract

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Keywords

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