TY - JOUR
T1 - Neuroprotective effects of Fructus Chebulae extracts on experimental models of cerebral ischemia
AU - Gaire, Bhakta Prasad
AU - Kim, Hocheol
PY - 2014/2
Y1 - 2014/2
N2 - OBJECTIVE: To investigate the neuroprotective effects of Fructus Chebulae extract using both in vivo and in vitro models of cerebral ischemia. METHODS: As an in vitro model, oxygen glucose deprivation followed by reoxygenation (OGD-R) and hydrogen peroxide (H2O2) induced cellular damage in rat pheochromocytoma (PC12) cells was used to investigate the neuroprotective effects of extract of Fructus Chebulae. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay was used to calculate cell survival. For in vivo, occlusion of left middle cerebral artery on rats was carried out as a focal cerebral ischemic model. RESULTS: Fructus Chebulae extract increases the PC12 cell survival against OGD-R and H2O2 by 68% and 91.4% respectively. Fructus Chebulae also decreases the cerebral infarct volume by 39% and extent of hemisphere swelling from 17% in control group to 10% in Fructus Chebulae treated group. CONCLUSION: Fructus Chebulae, as a traditional medicine, can rescue the neuronal cell death against ischemia related damage. The possible mechanism for the neuroprotection might be the inhibition of oxidative damages occurring after acute phase of cerebral ischemia.
AB - OBJECTIVE: To investigate the neuroprotective effects of Fructus Chebulae extract using both in vivo and in vitro models of cerebral ischemia. METHODS: As an in vitro model, oxygen glucose deprivation followed by reoxygenation (OGD-R) and hydrogen peroxide (H2O2) induced cellular damage in rat pheochromocytoma (PC12) cells was used to investigate the neuroprotective effects of extract of Fructus Chebulae. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay was used to calculate cell survival. For in vivo, occlusion of left middle cerebral artery on rats was carried out as a focal cerebral ischemic model. RESULTS: Fructus Chebulae extract increases the PC12 cell survival against OGD-R and H2O2 by 68% and 91.4% respectively. Fructus Chebulae also decreases the cerebral infarct volume by 39% and extent of hemisphere swelling from 17% in control group to 10% in Fructus Chebulae treated group. CONCLUSION: Fructus Chebulae, as a traditional medicine, can rescue the neuronal cell death against ischemia related damage. The possible mechanism for the neuroprotection might be the inhibition of oxidative damages occurring after acute phase of cerebral ischemia.
KW - Brain ischemia
KW - Fructus Chebulae
KW - Middle cerebral artery
KW - Neuroprotection
KW - Pheochromocytoma
UR - http://www.scopus.com/inward/record.url?scp=84893831766&partnerID=8YFLogxK
U2 - 10.1016/s0254-6272(14)60057-1
DO - 10.1016/s0254-6272(14)60057-1
M3 - Article
C2 - 25102694
AN - SCOPUS:84893831766
SN - 0255-2922
VL - 34
SP - 69
EP - 75
JO - Journal of Traditional Chinese Medicine
JF - Journal of Traditional Chinese Medicine
IS - 1
ER -