Neuroprotective effects of phlorotannin-rich extract from brown seaweed ecklonia cava on neuronal PC-12 and SH-SY5Y cells with oxidative stress

Jin Ah Nho, Yong Sub Shin, Ha Ram Jeong, Suengmok Cho, Ho Jin Heo, Gun Hee Kim, Dae Ok Kim

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Neurodegenerative disorders in the elderly are characterized by gradual loss of memory and cognitive function. Oxidative stress caused by reactive oxygen species is associated with progressive neuronal cell damage and death in Alzheimer's disease, one of the most common neurodegenerative disorders. An edible brown seaweed, Ecklonia cava, contains a variety of biologically active compounds such as phlorotannins. In this study, we comparatively evaluated the total phenolic content, antioxidant capacity, and neuroprotective effects of the phlorotannin-rich extract from E. cava (PEEC). The total phenolic content of PEEC and dieckol was 810.8 mg gallic acid equivalents (GAE)/g and 996.6 mg GAE/g, respectively. Antioxidant capacity of PEEC was 1,233.8 mg vitamin C equivalents (VCE)/g and 392.1 mg VCE/g determined using ABTS and DPPH assays, respectively, while those of dieckol were 2,238.4 mg VCE/g and 817.7 mg VCE/g. High-performance liquid chromatography results revealed 48.08 ± 0.67 mg dieckol/g of PEEC. PEEC had neuroprotective effects in pheochromocytoma (PC-12) and human neuroblastoma (SH-SY5Y) cells against H2O2- and AAPH-induced oxidative damage, partly due to reduced intracellular oxidative stress. PEEC treatment inhibited acetylcholinesterase and butyrylcholinesterase in a dose-dependent manner. Taken together, these findings suggest that PEEC is a good source of antioxidants and neuroprotective materials.

Original languageEnglish
Pages (from-to)359-367
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume30
Issue number3
DOIs
Publication statusPublished - 2020

Keywords

  • Cholinesterase
  • Dieckol
  • Neuron
  • Neuroprotection
  • Vitamin C equivalent antioxidant capacity

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