Newly combined method of molecularly imprinted solid-phase extraction with ELISA for rapid detection of clenbuterol in animal-tissue samples

Sulian Zheng; Suquan Song; Huo Lan; Guorun Qu; Rongxiu Li; Aibo Wu; Dabing Zhang

doi:10.1080/00032710802677134

Newly combined method of molecularly imprinted solid-phase extraction with ELISA for rapid detection of clenbuterol in animal-tissue samples

Sulian Zheng
, Suquan Song
, Huo Lan
, Guorun Qu
, Rongxiu Li
, Aibo Wu
, Dabing Zhang

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A new method using molecularly imprinted polymers (MIPs) as specific adsorbent materials coupled with ELISA analysis is being reported for the first time for the detection of clenbuterol (CLB) residue in the pig muscles. After optimization of the posttreatments, the total amount of template bleeding in the CLB MIPs was decreased to only 3.0 ng CLB/60 mg MIPs, which is 10 times lower than that of the previous report. Moreover, compared to the methods of C18-ELISA and single ELISA, the combined molecularly imprinted solid-phase extraction (MISPE)-ELISA exhibited high precision and robust accuracy for CLB at all three spiked levels of 0.5, 5.0, and 10.0 ng g-1.

Original language	English
Pages (from-to)	600-614
Number of pages	15
Journal	Analytical Letters
Volume	42
Issue number	3
DOIs	https://doi.org/10.1080/00032710802677134
Publication status	Published - Feb 2009

Bibliographical note

Funding Information:
This research was supported by the National ‘‘863’’ High-Tech Project (2006AA10Z438), National Key Project of Scientific and Technical Supporting Programs of China (2006BAK02A09), Shanghai Commission of Science and Technology (0652nm017, 07dz19508), and Shanghai Leading Academic Discipline Project (B205). We are grateful to Prof. Tom Hsiang (University of Guelph, Canada) for the technical language editing.

Keywords

Clenbuterol
ELISA
Molecularly imprinted polymers

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10.1080/00032710802677134

Cite this

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title = "Newly combined method of molecularly imprinted solid-phase extraction with ELISA for rapid detection of clenbuterol in animal-tissue samples",

abstract = "A new method using molecularly imprinted polymers (MIPs) as specific adsorbent materials coupled with ELISA analysis is being reported for the first time for the detection of clenbuterol (CLB) residue in the pig muscles. After optimization of the posttreatments, the total amount of template bleeding in the CLB MIPs was decreased to only 3.0 ng CLB/60 mg MIPs, which is 10 times lower than that of the previous report. Moreover, compared to the methods of C18-ELISA and single ELISA, the combined molecularly imprinted solid-phase extraction (MISPE)-ELISA exhibited high precision and robust accuracy for CLB at all three spiked levels of 0.5, 5.0, and 10.0 ng g-1.",

keywords = "Clenbuterol, ELISA, Molecularly imprinted polymers",

author = "Sulian Zheng and Suquan Song and Huo Lan and Guorun Qu and Rongxiu Li and Aibo Wu and Dabing Zhang",

note = "Funding Information: This research was supported by the National {\textquoteleft}{\textquoteleft}863{\textquoteright}{\textquoteright} High-Tech Project (2006AA10Z438), National Key Project of Scientific and Technical Supporting Programs of China (2006BAK02A09), Shanghai Commission of Science and Technology (0652nm017, 07dz19508), and Shanghai Leading Academic Discipline Project (B205). We are grateful to Prof. Tom Hsiang (University of Guelph, Canada) for the technical language editing.",

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doi = "10.1080/00032710802677134",

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volume = "42",

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T1 - Newly combined method of molecularly imprinted solid-phase extraction with ELISA for rapid detection of clenbuterol in animal-tissue samples

AU - Zheng, Sulian

AU - Song, Suquan

AU - Lan, Huo

AU - Qu, Guorun

AU - Li, Rongxiu

AU - Wu, Aibo

AU - Zhang, Dabing

N1 - Funding Information: This research was supported by the National ‘‘863’’ High-Tech Project (2006AA10Z438), National Key Project of Scientific and Technical Supporting Programs of China (2006BAK02A09), Shanghai Commission of Science and Technology (0652nm017, 07dz19508), and Shanghai Leading Academic Discipline Project (B205). We are grateful to Prof. Tom Hsiang (University of Guelph, Canada) for the technical language editing.

PY - 2009/2

Y1 - 2009/2

N2 - A new method using molecularly imprinted polymers (MIPs) as specific adsorbent materials coupled with ELISA analysis is being reported for the first time for the detection of clenbuterol (CLB) residue in the pig muscles. After optimization of the posttreatments, the total amount of template bleeding in the CLB MIPs was decreased to only 3.0 ng CLB/60 mg MIPs, which is 10 times lower than that of the previous report. Moreover, compared to the methods of C18-ELISA and single ELISA, the combined molecularly imprinted solid-phase extraction (MISPE)-ELISA exhibited high precision and robust accuracy for CLB at all three spiked levels of 0.5, 5.0, and 10.0 ng g-1.

AB - A new method using molecularly imprinted polymers (MIPs) as specific adsorbent materials coupled with ELISA analysis is being reported for the first time for the detection of clenbuterol (CLB) residue in the pig muscles. After optimization of the posttreatments, the total amount of template bleeding in the CLB MIPs was decreased to only 3.0 ng CLB/60 mg MIPs, which is 10 times lower than that of the previous report. Moreover, compared to the methods of C18-ELISA and single ELISA, the combined molecularly imprinted solid-phase extraction (MISPE)-ELISA exhibited high precision and robust accuracy for CLB at all three spiked levels of 0.5, 5.0, and 10.0 ng g-1.

KW - Clenbuterol

KW - ELISA

KW - Molecularly imprinted polymers

UR - https://www.scopus.com/pages/publications/60849096100

U2 - 10.1080/00032710802677134

DO - 10.1080/00032710802677134

M3 - Article

AN - SCOPUS:60849096100

SN - 0003-2719

VL - 42

SP - 600

EP - 614

JO - Analytical Letters

JF - Analytical Letters

IS - 3

ER -

Newly combined method of molecularly imprinted solid-phase extraction with ELISA for rapid detection of clenbuterol in animal-tissue samples

Abstract

Bibliographical note

Keywords

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