Niastella hibisci sp. nov., isolated from rhizosphere soil of mugunghwa, the Korean national flower

Zheng Fei Yan; Pei Lin; Yu Shuai Wang; Wei Gao; Chang Tian Li; Moo Chang Kook; Tae Hoo Yi

doi:10.1099/ijsem.0.001498

Niastella hibisci sp. nov., isolated from rhizosphere soil of mugunghwa, the Korean national flower

Zheng Fei Yan
, Pei Lin
, Yu Shuai Wang
, Wei Gao
, Chang Tian Li
, Moo Chang Kook
, Tae Hoo Yi

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A Gram-stain-negative, aerobic, non-motile, long rods with no flagellum strain, designated THGYS3.2.1^T, was isolated from rhizosphere soil of mugunghwa, collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40°C (optimum 28°C), at pH 6.0-8.0 (optimum pH 7.0) and with 0-1.0% NaCl (optimum 1.0%). The predominant menaquinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso-C_{12: 0}, iso-C_{14: 0}, iso-C_{15: 0}, anteiso-C_{15: 0}, iso-C_{15: 1} G, anteiso-C_{15: 1} A, C_{15: 0} 2-OH, C_{16: 0}, iso-C_{16: 0}, C_{16: 0} 3-OH, iso- C_{16: 0} 3-OH, iso-C_{16: 1} G, C_{17: 0} 2-OH, iso-C_{17: 0} 3-OH, C_{17: 1}w6c, C_{18: 3}w6c (6, 9, 12), C_{18: 0}, summed feature 3 (C_{16: 1}w7c and/or iso-C_{15: 0} 2-OH). The major polar lipids were phosphatidylmethylethanolamine), phosphatidylethanolamine, five unidentified aminolipids and three unidentified lipids. The DNA G+C content of strain THG-YS3.2.1^T was 45.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THGYS3.2.1^T were Niastella populi KCTC 22560^T (98.7% 16S rRNA gene sequence similarity), Niastella gongjuensis KACC 17399^T (96.9%), Niastella vici KCTC 42474^T (96.2%), Niastella yeongjuensis KACC 11466^T (95.5%) and Niastella koreensis KACC 11465^T (95.1%). DNA– DNA hybridization values between strain THG-YS3.2.1^T and N. populi KCTC 22560^T, N. gongjuensis KACC 17399^T, N. vici KCTC 42474^T, N. yeongjuensis KACC 11466^T and N. koreensis KACC 11465^Twere 55.8±1.0, 39.5±0.5, 35.2±0.1, 17.6±0.3 and 12.5±1.2%, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.2.1^T represents a novel species of the genus Niastella, for which the name Niastella hibisci sp. nov. is proposed. The type strain is THG-YS3.2.1^T (=KCTC 52084^T=CCTCC AB 2015356^T).

Original language	English
Pages (from-to)	5218-5222
Number of pages	5
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	66
Issue number	12
DOIs	https://doi.org/10.1099/ijsem.0.001498
Publication status	Published - Dec 2016

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© 2016 IUMS.

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10.1099/ijsem.0.001498

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@article{53a0453ad5b849f3a880421a4077e19f,

title = "Niastella hibisci sp. nov., isolated from rhizosphere soil of mugunghwa, the Korean national flower",

abstract = "A Gram-stain-negative, aerobic, non-motile, long rods with no flagellum strain, designated THGYS3.2.1T, was isolated from rhizosphere soil of mugunghwa, collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40°C (optimum 28°C), at pH 6.0-8.0 (optimum pH 7.0) and with 0-1.0\% NaCl (optimum 1.0\%). The predominant menaquinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso-C12: 0, iso-C14: 0, iso-C15: 0, anteiso-C15: 0, iso-C15: 1 G, anteiso-C15: 1 A, C15: 0 2-OH, C16: 0, iso-C16: 0, C16: 0 3-OH, iso- C16: 0 3-OH, iso-C16: 1 G, C17: 0 2-OH, iso-C17: 0 3-OH, C17: 1w6c, C18: 3w6c (6, 9, 12), C18: 0, summed feature 3 (C16: 1w7c and/or iso-C15: 0 2-OH). The major polar lipids were phosphatidylmethylethanolamine), phosphatidylethanolamine, five unidentified aminolipids and three unidentified lipids. The DNA G+C content of strain THG-YS3.2.1T was 45.3 mol\%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THGYS3.2.1T were Niastella populi KCTC 22560T (98.7\% 16S rRNA gene sequence similarity), Niastella gongjuensis KACC 17399T (96.9\%), Niastella vici KCTC 42474T (96.2\%), Niastella yeongjuensis KACC 11466T (95.5\%) and Niastella koreensis KACC 11465T (95.1\%). DNA– DNA hybridization values between strain THG-YS3.2.1T and N. populi KCTC 22560T, N. gongjuensis KACC 17399T, N. vici KCTC 42474T, N. yeongjuensis KACC 11466T and N. koreensis KACC 11465Twere 55.8±1.0, 39.5±0.5, 35.2±0.1, 17.6±0.3 and 12.5±1.2\%, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.2.1T represents a novel species of the genus Niastella, for which the name Niastella hibisci sp. nov. is proposed. The type strain is THG-YS3.2.1T (=KCTC 52084T=CCTCC AB 2015356T).",

author = "Yan, \{Zheng Fei\} and Pei Lin and Wang, \{Yu Shuai\} and Wei Gao and Li, \{Chang Tian\} and Kook, \{Moo Chang\} and Yi, \{Tae Hoo\}",

note = "Publisher Copyright: {\textcopyright} 2016 IUMS.",

year = "2016",

month = dec,

doi = "10.1099/ijsem.0.001498",

language = "English",

volume = "66",

pages = "5218--5222",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

publisher = "Microbiology Society",

number = "12",

}

TY - JOUR

T1 - Niastella hibisci sp. nov., isolated from rhizosphere soil of mugunghwa, the Korean national flower

AU - Yan, Zheng Fei

AU - Lin, Pei

AU - Wang, Yu Shuai

AU - Gao, Wei

AU - Li, Chang Tian

AU - Kook, Moo Chang

AU - Yi, Tae Hoo

PY - 2016/12

Y1 - 2016/12

N2 - A Gram-stain-negative, aerobic, non-motile, long rods with no flagellum strain, designated THGYS3.2.1T, was isolated from rhizosphere soil of mugunghwa, collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40°C (optimum 28°C), at pH 6.0-8.0 (optimum pH 7.0) and with 0-1.0% NaCl (optimum 1.0%). The predominant menaquinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso-C12: 0, iso-C14: 0, iso-C15: 0, anteiso-C15: 0, iso-C15: 1 G, anteiso-C15: 1 A, C15: 0 2-OH, C16: 0, iso-C16: 0, C16: 0 3-OH, iso- C16: 0 3-OH, iso-C16: 1 G, C17: 0 2-OH, iso-C17: 0 3-OH, C17: 1w6c, C18: 3w6c (6, 9, 12), C18: 0, summed feature 3 (C16: 1w7c and/or iso-C15: 0 2-OH). The major polar lipids were phosphatidylmethylethanolamine), phosphatidylethanolamine, five unidentified aminolipids and three unidentified lipids. The DNA G+C content of strain THG-YS3.2.1T was 45.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THGYS3.2.1T were Niastella populi KCTC 22560T (98.7% 16S rRNA gene sequence similarity), Niastella gongjuensis KACC 17399T (96.9%), Niastella vici KCTC 42474T (96.2%), Niastella yeongjuensis KACC 11466T (95.5%) and Niastella koreensis KACC 11465T (95.1%). DNA– DNA hybridization values between strain THG-YS3.2.1T and N. populi KCTC 22560T, N. gongjuensis KACC 17399T, N. vici KCTC 42474T, N. yeongjuensis KACC 11466T and N. koreensis KACC 11465Twere 55.8±1.0, 39.5±0.5, 35.2±0.1, 17.6±0.3 and 12.5±1.2%, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.2.1T represents a novel species of the genus Niastella, for which the name Niastella hibisci sp. nov. is proposed. The type strain is THG-YS3.2.1T (=KCTC 52084T=CCTCC AB 2015356T).

AB - A Gram-stain-negative, aerobic, non-motile, long rods with no flagellum strain, designated THGYS3.2.1T, was isolated from rhizosphere soil of mugunghwa, collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40°C (optimum 28°C), at pH 6.0-8.0 (optimum pH 7.0) and with 0-1.0% NaCl (optimum 1.0%). The predominant menaquinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso-C12: 0, iso-C14: 0, iso-C15: 0, anteiso-C15: 0, iso-C15: 1 G, anteiso-C15: 1 A, C15: 0 2-OH, C16: 0, iso-C16: 0, C16: 0 3-OH, iso- C16: 0 3-OH, iso-C16: 1 G, C17: 0 2-OH, iso-C17: 0 3-OH, C17: 1w6c, C18: 3w6c (6, 9, 12), C18: 0, summed feature 3 (C16: 1w7c and/or iso-C15: 0 2-OH). The major polar lipids were phosphatidylmethylethanolamine), phosphatidylethanolamine, five unidentified aminolipids and three unidentified lipids. The DNA G+C content of strain THG-YS3.2.1T was 45.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THGYS3.2.1T were Niastella populi KCTC 22560T (98.7% 16S rRNA gene sequence similarity), Niastella gongjuensis KACC 17399T (96.9%), Niastella vici KCTC 42474T (96.2%), Niastella yeongjuensis KACC 11466T (95.5%) and Niastella koreensis KACC 11465T (95.1%). DNA– DNA hybridization values between strain THG-YS3.2.1T and N. populi KCTC 22560T, N. gongjuensis KACC 17399T, N. vici KCTC 42474T, N. yeongjuensis KACC 11466T and N. koreensis KACC 11465Twere 55.8±1.0, 39.5±0.5, 35.2±0.1, 17.6±0.3 and 12.5±1.2%, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.2.1T represents a novel species of the genus Niastella, for which the name Niastella hibisci sp. nov. is proposed. The type strain is THG-YS3.2.1T (=KCTC 52084T=CCTCC AB 2015356T).

UR - https://www.scopus.com/pages/publications/85010332885

U2 - 10.1099/ijsem.0.001498

DO - 10.1099/ijsem.0.001498

M3 - Article

C2 - 27638144

AN - SCOPUS:85010332885

SN - 1466-5026

VL - 66

SP - 5218

EP - 5222

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 12

ER -

Niastella hibisci sp. nov., isolated from rhizosphere soil of mugunghwa, the Korean national flower

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