(Nitrilotriacetic acid)-end-functionalized polystyrenes synthesized by ATRP

Mohammad Abdul Kadir; Hong Y. Cho; Bong Soo Kim; Young Rok Kim; Sun Gu Lee; Unyong Jeong; Hyun Jong Paik

doi:10.1021/bk-2012-1101.ch020

(Nitrilotriacetic acid)-end-functionalized polystyrenes synthesized by ATRP

Mohammad Abdul Kadir, Hong Y. Cho, Bong Soo Kim, Young Rok Kim, Sun Gu Lee, Unyong Jeong, Hyun Jong Paik

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The synthesis of nitrilotriacetic acid end-functionalized polystyrenes (NTA-PS) using t-butyl protected NTA initiators via atom transfer radial polymerization (ATRP) of styrene is described. The protected t-butyl group is subsequently removed at the α-chain end of polystyrene. After complexation of NTA chain ends of the polystyrenes with Ni²⁺ to produce Ni-NTA-PS, conjugation with (six histidine-tagged green fluorescent protein) His₆-GFP via Ni²⁺/histidine (His) interaction and the self-assembly of the resulted bioconjugate are studied. Highly mesoporous NTA-PS fibers are also produced by taking advantage of interpenetrating phase separation between PS and poly(ethylene oxide) (PEO) during electrospinning. The specific interaction of Ni²⁺-complexed NTA-PS fibers with His enables to immobilize and purify only target proteins from highly heterogeneous protein mixtures.

Original language	English
Title of host publication	Progress in Controlled Radical Polymerization
Subtitle of host publication	Materials and Applications
Publisher	American Chemical Society
Pages	303-314
Number of pages	12
ISBN (Print)	9780841227576
DOIs	https://doi.org/10.1021/bk-2012-1101.ch020
Publication status	Published - 2012

Publication series

Name	ACS Symposium Series
Volume	1101
ISSN (Print)	0097-6156
ISSN (Electronic)	1947-5918

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10.1021/bk-2012-1101.ch020

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title = "(Nitrilotriacetic acid)-end-functionalized polystyrenes synthesized by ATRP",

abstract = "The synthesis of nitrilotriacetic acid end-functionalized polystyrenes (NTA-PS) using t-butyl protected NTA initiators via atom transfer radial polymerization (ATRP) of styrene is described. The protected t-butyl group is subsequently removed at the α-chain end of polystyrene. After complexation of NTA chain ends of the polystyrenes with Ni2+ to produce Ni-NTA-PS, conjugation with (six histidine-tagged green fluorescent protein) His6-GFP via Ni2+/histidine (His) interaction and the self-assembly of the resulted bioconjugate are studied. Highly mesoporous NTA-PS fibers are also produced by taking advantage of interpenetrating phase separation between PS and poly(ethylene oxide) (PEO) during electrospinning. The specific interaction of Ni2+-complexed NTA-PS fibers with His enables to immobilize and purify only target proteins from highly heterogeneous protein mixtures.",

author = "Kadir, {Mohammad Abdul} and Cho, {Hong Y.} and Kim, {Bong Soo} and Kim, {Young Rok} and Lee, {Sun Gu} and Unyong Jeong and Paik, {Hyun Jong}",

year = "2012",

doi = "10.1021/bk-2012-1101.ch020",

language = "English",

isbn = "9780841227576",

series = "ACS Symposium Series",

publisher = "American Chemical Society",

pages = "303--314",

booktitle = "Progress in Controlled Radical Polymerization",

address = "United States",

}

(Nitrilotriacetic acid)-end-functionalized polystyrenes synthesized by ATRP. / Kadir, Mohammad Abdul; Cho, Hong Y.; Kim, Bong Soo et al.
Progress in Controlled Radical Polymerization: Materials and Applications. American Chemical Society, 2012. p. 303-314 (ACS Symposium Series; Vol. 1101).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - (Nitrilotriacetic acid)-end-functionalized polystyrenes synthesized by ATRP

AU - Kadir, Mohammad Abdul

AU - Cho, Hong Y.

AU - Kim, Bong Soo

AU - Kim, Young Rok

AU - Lee, Sun Gu

AU - Jeong, Unyong

AU - Paik, Hyun Jong

PY - 2012

Y1 - 2012

N2 - The synthesis of nitrilotriacetic acid end-functionalized polystyrenes (NTA-PS) using t-butyl protected NTA initiators via atom transfer radial polymerization (ATRP) of styrene is described. The protected t-butyl group is subsequently removed at the α-chain end of polystyrene. After complexation of NTA chain ends of the polystyrenes with Ni2+ to produce Ni-NTA-PS, conjugation with (six histidine-tagged green fluorescent protein) His6-GFP via Ni2+/histidine (His) interaction and the self-assembly of the resulted bioconjugate are studied. Highly mesoporous NTA-PS fibers are also produced by taking advantage of interpenetrating phase separation between PS and poly(ethylene oxide) (PEO) during electrospinning. The specific interaction of Ni2+-complexed NTA-PS fibers with His enables to immobilize and purify only target proteins from highly heterogeneous protein mixtures.

AB - The synthesis of nitrilotriacetic acid end-functionalized polystyrenes (NTA-PS) using t-butyl protected NTA initiators via atom transfer radial polymerization (ATRP) of styrene is described. The protected t-butyl group is subsequently removed at the α-chain end of polystyrene. After complexation of NTA chain ends of the polystyrenes with Ni2+ to produce Ni-NTA-PS, conjugation with (six histidine-tagged green fluorescent protein) His6-GFP via Ni2+/histidine (His) interaction and the self-assembly of the resulted bioconjugate are studied. Highly mesoporous NTA-PS fibers are also produced by taking advantage of interpenetrating phase separation between PS and poly(ethylene oxide) (PEO) during electrospinning. The specific interaction of Ni2+-complexed NTA-PS fibers with His enables to immobilize and purify only target proteins from highly heterogeneous protein mixtures.

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U2 - 10.1021/bk-2012-1101.ch020

DO - 10.1021/bk-2012-1101.ch020

M3 - Conference contribution

AN - SCOPUS:84905643827

SN - 9780841227576

T3 - ACS Symposium Series

SP - 303

EP - 314

BT - Progress in Controlled Radical Polymerization

PB - American Chemical Society

ER -

(Nitrilotriacetic acid)-end-functionalized polystyrenes synthesized by ATRP

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