Observation of angiotensin II-induced changes in fixed and live mesangial cells by atomic force microscopy

Gi Ja Lee; Eun Jin Park; Samjin Choi; Jeong Hoon Park; Kyung Hwan Jeong; Kyung Sook Kim; Sang Ho Lee; Hun Kuk Park

doi:10.1016/j.micron.2009.10.012

Observation of angiotensin II-induced changes in fixed and live mesangial cells by atomic force microscopy

Gi Ja Lee, Eun Jin Park, Samjin Choi, Jeong Hoon Park, Kyung Hwan Jeong, Kyung Sook Kim, Sang Ho Lee, Hun Kuk Park

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Glomerular mesangial cells (MCs) are centrally located in the glomerulus. MCs control not only glomerular filtration, but also the response to local injury, including cell proliferation and basement membrane remodeling. Angiotensin II (Ang II) plays an important role in kidney function regulation, and participates in the progression of renal damage, as well as mesangial injury. However, studies on Ang II effects on MCs have used indirect methods, such as gene and protein expression after MC injury. In this study, we visually observed structural and mechanical changes to MC after Ang II treatment using atomic force microscopy (AFM). We obtained AFM topography and deflection images of live MCs, as well as fixed MCs in liquid, before and after Ang II treatment. Real-time imaging showed the dynamic movement of live MCs induced by Ang II. Changes in MC elastic property after Ang II treatment were measured using force-distance curves. AFM images of fixed and live MCs showed that cells contracted after Ang II exposure, with the nucleus height increasing within 20 min of Ang II stimulation. Force-distance analysis showed that Ang II caused MCs to stiffen (p < 0.0001). In conclusion, we demonstrated that AFM is an effective tool for real-time monitoring of live cell responses to drugs and stimuli.

Original language	English
Pages (from-to)	220-226
Number of pages	7
Journal	Micron
Volume	41
Issue number	3
DOIs	https://doi.org/10.1016/j.micron.2009.10.012
Publication status	Published - Apr 2010

Keywords

Angiotensin II
Atomic force microscopy
Force-distance curve
Live cells
Mesangial cells
Real-time monitoring

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10.1016/j.micron.2009.10.012

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title = "Observation of angiotensin II-induced changes in fixed and live mesangial cells by atomic force microscopy",

abstract = "Glomerular mesangial cells (MCs) are centrally located in the glomerulus. MCs control not only glomerular filtration, but also the response to local injury, including cell proliferation and basement membrane remodeling. Angiotensin II (Ang II) plays an important role in kidney function regulation, and participates in the progression of renal damage, as well as mesangial injury. However, studies on Ang II effects on MCs have used indirect methods, such as gene and protein expression after MC injury. In this study, we visually observed structural and mechanical changes to MC after Ang II treatment using atomic force microscopy (AFM). We obtained AFM topography and deflection images of live MCs, as well as fixed MCs in liquid, before and after Ang II treatment. Real-time imaging showed the dynamic movement of live MCs induced by Ang II. Changes in MC elastic property after Ang II treatment were measured using force-distance curves. AFM images of fixed and live MCs showed that cells contracted after Ang II exposure, with the nucleus height increasing within 20 min of Ang II stimulation. Force-distance analysis showed that Ang II caused MCs to stiffen (p < 0.0001). In conclusion, we demonstrated that AFM is an effective tool for real-time monitoring of live cell responses to drugs and stimuli.",

keywords = "Angiotensin II, Atomic force microscopy, Force-distance curve, Live cells, Mesangial cells, Real-time monitoring",

author = "Lee, {Gi Ja} and Park, {Eun Jin} and Samjin Choi and Park, {Jeong Hoon} and Jeong, {Kyung Hwan} and Kim, {Kyung Sook} and Lee, {Sang Ho} and Park, {Hun Kuk}",

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AU - Jeong, Kyung Hwan

AU - Kim, Kyung Sook

AU - Lee, Sang Ho

AU - Park, Hun Kuk

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N2 - Glomerular mesangial cells (MCs) are centrally located in the glomerulus. MCs control not only glomerular filtration, but also the response to local injury, including cell proliferation and basement membrane remodeling. Angiotensin II (Ang II) plays an important role in kidney function regulation, and participates in the progression of renal damage, as well as mesangial injury. However, studies on Ang II effects on MCs have used indirect methods, such as gene and protein expression after MC injury. In this study, we visually observed structural and mechanical changes to MC after Ang II treatment using atomic force microscopy (AFM). We obtained AFM topography and deflection images of live MCs, as well as fixed MCs in liquid, before and after Ang II treatment. Real-time imaging showed the dynamic movement of live MCs induced by Ang II. Changes in MC elastic property after Ang II treatment were measured using force-distance curves. AFM images of fixed and live MCs showed that cells contracted after Ang II exposure, with the nucleus height increasing within 20 min of Ang II stimulation. Force-distance analysis showed that Ang II caused MCs to stiffen (p < 0.0001). In conclusion, we demonstrated that AFM is an effective tool for real-time monitoring of live cell responses to drugs and stimuli.

AB - Glomerular mesangial cells (MCs) are centrally located in the glomerulus. MCs control not only glomerular filtration, but also the response to local injury, including cell proliferation and basement membrane remodeling. Angiotensin II (Ang II) plays an important role in kidney function regulation, and participates in the progression of renal damage, as well as mesangial injury. However, studies on Ang II effects on MCs have used indirect methods, such as gene and protein expression after MC injury. In this study, we visually observed structural and mechanical changes to MC after Ang II treatment using atomic force microscopy (AFM). We obtained AFM topography and deflection images of live MCs, as well as fixed MCs in liquid, before and after Ang II treatment. Real-time imaging showed the dynamic movement of live MCs induced by Ang II. Changes in MC elastic property after Ang II treatment were measured using force-distance curves. AFM images of fixed and live MCs showed that cells contracted after Ang II exposure, with the nucleus height increasing within 20 min of Ang II stimulation. Force-distance analysis showed that Ang II caused MCs to stiffen (p < 0.0001). In conclusion, we demonstrated that AFM is an effective tool for real-time monitoring of live cell responses to drugs and stimuli.

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KW - Real-time monitoring

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Observation of angiotensin II-induced changes in fixed and live mesangial cells by atomic force microscopy

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