Optical measurement of synaptic vesicle fusion and its inhibition by inositol pyrophosphate in primary cultured hippocampal neurons

Sung Hyun Kim

doi:10.1007/978-1-0716-0167-9_11

Optical measurement of synaptic vesicle fusion and its inhibition by inositol pyrophosphate in primary cultured hippocampal neurons

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Optical molecular and cellular imaging has provided considerable information in various life science research fields. In particular, pHluorin-based assays allow us to specifically investigate presynaptic physiological processes, such as synaptic transmission and synaptic retrieval. Here, I describe quantitative synaptic transmission in primary cultured hippocampal neurons using a vGlut-pHluorin system combined with a high-fidelity optical imaging system and its regulation by inositol pyrophosphate.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	131-135
Number of pages	5
DOIs	https://doi.org/10.1007/978-1-0716-0167-9_11
Publication status	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2091
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

Keywords

IP6K
Inositol pyrophosphate
Synaptic transmission
Synaptic vesicle
vGlut-pHluorin (vG-pH)

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10.1007/978-1-0716-0167-9_11

Cite this

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KW - Inositol pyrophosphate

KW - Synaptic transmission

KW - Synaptic vesicle

KW - vGlut-pHluorin (vG-pH)

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Optical measurement of synaptic vesicle fusion and its inhibition by inositol pyrophosphate in primary cultured hippocampal neurons

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