Optimal cell density and multiplicity of infection for the propagation of human rotavirus in monkey kidney cells

Kyung Hwa Chang; Chang Ho Park; Jong Hwa Park; In Sik Chung

doi:10.1023/A:1008949726629

Optimal cell density and multiplicity of infection for the propagation of human rotavirus in monkey kidney cells

Kyung Hwa Chang
, Chang Ho Park
, Jong Hwa Park
, In Sik Chung

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The human rotavirus titer was optimal at an infection cell density of about 4-8 x 10⁴ cells cm^-2 in monkey kidney cell cultures. The highest viral titers (3.8 x 10⁷ TCID₅₀ ml^-1 and 3.7 x 10⁷ TCID₅₀ ml^-1) were obtained at an multiplicity of infection of 0.05 in well plate and T-flask, respectively.

Original language	English
Pages (from-to)	757-759
Number of pages	3
Journal	Biotechnology Techniques
Volume	13
Issue number	11
DOIs	https://doi.org/10.1023/A:1008949726629
Publication status	Published - 1999

Bibliographical note

Funding Information:
This work was supported by a grant from the Academic Research Promotion Fund (Biochemical Engineering) from the Ministry of Education of the Republic of Korea (1998).

Keywords

Cell density
Human rotavirus
Infection
Multiplicity
Propagation

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10.1023/A:1008949726629

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title = "Optimal cell density and multiplicity of infection for the propagation of human rotavirus in monkey kidney cells",

abstract = "The human rotavirus titer was optimal at an infection cell density of about 4-8 x 104 cells cm-2 in monkey kidney cell cultures. The highest viral titers (3.8 x 107 TCID50 ml-1 and 3.7 x 107 TCID50 ml-1) were obtained at an multiplicity of infection of 0.05 in well plate and T-flask, respectively.",

keywords = "Cell density, Human rotavirus, Infection, Multiplicity, Propagation",

author = "Chang, \{Kyung Hwa\} and Park, \{Chang Ho\} and Park, \{Jong Hwa\} and Chung, \{In Sik\}",

note = "Funding Information: This work was supported by a grant from the Academic Research Promotion Fund (Biochemical Engineering) from the Ministry of Education of the Republic of Korea (1998).",

year = "1999",

doi = "10.1023/A:1008949726629",

language = "English",

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TY - JOUR

T1 - Optimal cell density and multiplicity of infection for the propagation of human rotavirus in monkey kidney cells

AU - Chang, Kyung Hwa

AU - Park, Chang Ho

AU - Park, Jong Hwa

AU - Chung, In Sik

N1 - Funding Information: This work was supported by a grant from the Academic Research Promotion Fund (Biochemical Engineering) from the Ministry of Education of the Republic of Korea (1998).

PY - 1999

Y1 - 1999

N2 - The human rotavirus titer was optimal at an infection cell density of about 4-8 x 104 cells cm-2 in monkey kidney cell cultures. The highest viral titers (3.8 x 107 TCID50 ml-1 and 3.7 x 107 TCID50 ml-1) were obtained at an multiplicity of infection of 0.05 in well plate and T-flask, respectively.

AB - The human rotavirus titer was optimal at an infection cell density of about 4-8 x 104 cells cm-2 in monkey kidney cell cultures. The highest viral titers (3.8 x 107 TCID50 ml-1 and 3.7 x 107 TCID50 ml-1) were obtained at an multiplicity of infection of 0.05 in well plate and T-flask, respectively.

KW - Cell density

KW - Human rotavirus

KW - Infection

KW - Multiplicity

KW - Propagation

UR - https://www.scopus.com/pages/publications/0032801375

U2 - 10.1023/A:1008949726629

DO - 10.1023/A:1008949726629

M3 - Article

AN - SCOPUS:0032801375

SN - 0951-208X

VL - 13

SP - 757

EP - 759

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 11

ER -

Optimal cell density and multiplicity of infection for the propagation of human rotavirus in monkey kidney cells

Abstract

Bibliographical note

Keywords

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