Osteogenic commitment of human induced pluripotent stem cell-derived mesenchymal progenitor-like cells on biomimetic scaffolds

In this study, mesenchymal progenitor-like cells (MPLCs) were derived from human induced pluripotent stem cells (hiPSCs) by retinoic acid (RA) treatment. In particular, RA induced hiPSC-derived MPLCs were able to efficiently committed into osteogenic lineage cells. In addition, hiPSC-MPLCs were seeded onto three-dimensional biomimetic scaffolds composed of hydroxyapatite and poly(. l-lactic acid)/poly(lactic-co-glycolide) for assessing osteoinductivity, and enhanced osteogenic activities were perceived in RA-treated cells in vitro and in vivo. In conclusion, addition of RA increased the osteogenic commitment potential of hiPSCs-MPLCs and further applications with biomimetic composite scaffolds showed a promising combination for bone tissue engineering applications.