Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children

You Kyung Shin; Ji Won Choi; Se Young Oh; Jayong Chung

doi:10.4163/jnh.2015.48.6.468

Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children

You Kyung Shin, Ji Won Choi, Se Young Oh, Jayong Chung

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Purpose: Genetic polymorphisms in antioxidant defense and detoxification genes may modulate the levels of oxidative stress biomarkers. Methods: A total of 301 healthy preschool-aged children in the Seoul and Kyung-gi areas were recruited. DNA was extracted from blood for genotyping of manganese superoxide dismutase (Mn-SOD) Val16Ala, glutathione S-transferase (GST) P1 Ile105Val, GSTT1 present/null, and GSTM1 present/null polymorphisms by PCR-restriction fragment length polymorphism or multiplex PCR analyses. In addition to a questionnaire survey, the levels of urinary 8-hydroxyl-2-deoxiguanosine (8-OHdG) and plasma malondialdehyde (MDA) were measured by ELISA. Results: Significantly higher urinary 8-OHdG concentrations were observed in GSTP1 Ile/Val + Val/Val genotype (p = 0.030), and tended to be higher in Mn-SOD Val/Val genotype (p = 0.065). On the other hand, exposure to environmental tobacco smoking (ETS) and interaction between ETS and gene polymorphisms did not significantly influence either urinary 8-OHdG concentrations or serum MDA. Conclusion: Based on our findings, GSTP1 Ile/Val gene polymorphisms might modulate the levels of oxidative stress biomarkers in healthy preschool children.

Original language	English
Pages (from-to)	468-475
Number of pages	8
Journal	Journal of Nutrition and Health
Volume	48
Issue number	6
DOIs	https://doi.org/10.4163/jnh.2015.48.6.468
Publication status	Published - Dec 2015

Bibliographical note

Publisher Copyright:
© 2015 The Korean Nutrition Society.

Keywords

8-Hydroyxyl-2-deoxyguanosine
Genetic polymorphism
Glutathione S-transferase
Mn-superoxide dismutase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4163/jnh.2015.48.6.468

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title = "Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children",

abstract = "Purpose: Genetic polymorphisms in antioxidant defense and detoxification genes may modulate the levels of oxidative stress biomarkers. Methods: A total of 301 healthy preschool-aged children in the Seoul and Kyung-gi areas were recruited. DNA was extracted from blood for genotyping of manganese superoxide dismutase (Mn-SOD) Val16Ala, glutathione S-transferase (GST) P1 Ile105Val, GSTT1 present/null, and GSTM1 present/null polymorphisms by PCR-restriction fragment length polymorphism or multiplex PCR analyses. In addition to a questionnaire survey, the levels of urinary 8-hydroxyl-2-deoxiguanosine (8-OHdG) and plasma malondialdehyde (MDA) were measured by ELISA. Results: Significantly higher urinary 8-OHdG concentrations were observed in GSTP1 Ile/Val + Val/Val genotype (p = 0.030), and tended to be higher in Mn-SOD Val/Val genotype (p = 0.065). On the other hand, exposure to environmental tobacco smoking (ETS) and interaction between ETS and gene polymorphisms did not significantly influence either urinary 8-OHdG concentrations or serum MDA. Conclusion: Based on our findings, GSTP1 Ile/Val gene polymorphisms might modulate the levels of oxidative stress biomarkers in healthy preschool children.",

keywords = "8-Hydroyxyl-2-deoxyguanosine, Genetic polymorphism, Glutathione S-transferase, Mn-superoxide dismutase",

author = "Shin, {You Kyung} and Choi, {Ji Won} and Oh, {Se Young} and Jayong Chung",

note = "Publisher Copyright: {\textcopyright} 2015 The Korean Nutrition Society.",

year = "2015",

month = dec,

doi = "10.4163/jnh.2015.48.6.468",

language = "English",

volume = "48",

pages = "468--475",

journal = "Journal of Nutrition and Health",

issn = "2288-3886",

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T1 - Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children

AU - Shin, You Kyung

AU - Choi, Ji Won

AU - Oh, Se Young

AU - Chung, Jayong

PY - 2015/12

Y1 - 2015/12

N2 - Purpose: Genetic polymorphisms in antioxidant defense and detoxification genes may modulate the levels of oxidative stress biomarkers. Methods: A total of 301 healthy preschool-aged children in the Seoul and Kyung-gi areas were recruited. DNA was extracted from blood for genotyping of manganese superoxide dismutase (Mn-SOD) Val16Ala, glutathione S-transferase (GST) P1 Ile105Val, GSTT1 present/null, and GSTM1 present/null polymorphisms by PCR-restriction fragment length polymorphism or multiplex PCR analyses. In addition to a questionnaire survey, the levels of urinary 8-hydroxyl-2-deoxiguanosine (8-OHdG) and plasma malondialdehyde (MDA) were measured by ELISA. Results: Significantly higher urinary 8-OHdG concentrations were observed in GSTP1 Ile/Val + Val/Val genotype (p = 0.030), and tended to be higher in Mn-SOD Val/Val genotype (p = 0.065). On the other hand, exposure to environmental tobacco smoking (ETS) and interaction between ETS and gene polymorphisms did not significantly influence either urinary 8-OHdG concentrations or serum MDA. Conclusion: Based on our findings, GSTP1 Ile/Val gene polymorphisms might modulate the levels of oxidative stress biomarkers in healthy preschool children.

AB - Purpose: Genetic polymorphisms in antioxidant defense and detoxification genes may modulate the levels of oxidative stress biomarkers. Methods: A total of 301 healthy preschool-aged children in the Seoul and Kyung-gi areas were recruited. DNA was extracted from blood for genotyping of manganese superoxide dismutase (Mn-SOD) Val16Ala, glutathione S-transferase (GST) P1 Ile105Val, GSTT1 present/null, and GSTM1 present/null polymorphisms by PCR-restriction fragment length polymorphism or multiplex PCR analyses. In addition to a questionnaire survey, the levels of urinary 8-hydroxyl-2-deoxiguanosine (8-OHdG) and plasma malondialdehyde (MDA) were measured by ELISA. Results: Significantly higher urinary 8-OHdG concentrations were observed in GSTP1 Ile/Val + Val/Val genotype (p = 0.030), and tended to be higher in Mn-SOD Val/Val genotype (p = 0.065). On the other hand, exposure to environmental tobacco smoking (ETS) and interaction between ETS and gene polymorphisms did not significantly influence either urinary 8-OHdG concentrations or serum MDA. Conclusion: Based on our findings, GSTP1 Ile/Val gene polymorphisms might modulate the levels of oxidative stress biomarkers in healthy preschool children.

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KW - Genetic polymorphism

KW - Glutathione S-transferase

KW - Mn-superoxide dismutase

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Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children

Abstract

Bibliographical note

Keywords

UN SDGs

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