Paracoccus hibiscisoli sp. Nov., isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus)

A Gram-reaction-negative, aerobic, non-motile, short-rod-shaped bacterium (THG-T2.31^T) was isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus). Growth occurred at 10–35 °C (optimum 28 °C), at pH 5.0–8.0 (optimum pH 7.0) and with 0–4.0%NaCl (optimum 1.0 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T2.31^T were identified as Paracoccus marcusii DSM 11574^T (98.4 %), Paracoccus haeundaensis BC74171^T (98.3 %), Paracoccus carotinifaciens E-396^T (98.3 %), Paracoccus aestuarii B7^T (97.3 %) and Paracoccus seriniphilus MBT-A4^T (97.0 %); levels of similarity with the type strains of other species of the genus Paracoccus were lower than 97.0 %. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified aminolipid and two unidentified phospholipids. The major fatty acids were C_{16 :0}, C_18:0, C_10:0 3-OH, and C_18:1ω7c. The quinone was ubiquinone-10 (Q-10). The DNA G+C content of strain THG-T2.31^T was 69.1 mol%. DNA–DNA hybridization values between strain THG-T2.31^T and P. marcusii DSM 11574^T, P. haeundaensis BC74171^T, P. carotinifaciens E-396^T, P. aestuarii B7^T and P. seriniphilus MBT-A4^T were 38.9% (34.9 %, reciprocal analysis), 29.1% (23.5 %), 28.0% (19.7 %), 18.9% (9.3) and 13.1% (6.2 %). On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-T2.31^T represents a novel species of the genus Paracoccus, for which the name Paracoccus hibiscisoli sp. nov. is proposed. The type strain is THG-T2.31^T (=KACC 18933^T=CCTCC AB 2016182^T).