Abstract
In our search for peroxisome proliferator-activated receptor (PPAR) agonists, five undescribed compounds, namely two acyclic diterpenes (1 and 2; cladopsol A and cladopsol B), two sesquiterpenes (3 and 4; cladopsol C and cladopsol D), and one C21-ecdysteroid (5; cladopsol E), and 15 known compounds were isolated from the jellyfish-derived fungus – Cladosporium oxysporum. The structures of the undescribed compounds were defined using UV, NMR, HR-ESI-MS, and electronic circular dichroism (ECD) spectroscopy and a modified Mosher's method. Luciferase reporter assay and docking analysis suggested that cladopsol B may function as a PPAR-γ partial agonist with a potential antidiabetic lead which may evade the side effects of full agonists. Moreover, cladopsol B stimulated glucose uptake in HepG2 cells with an efficacy comparable to that of rosiglitazone, but with less side effect induced by lipid accumulation in 3T3-L1 cells. Therefore, cladopsol B could serve as a molecular skeleton in a study of advanced antidiabetic lead with less side effect.
| Original language | English |
|---|---|
| Article number | e202300851 |
| Journal | Chemistry and Biodiversity |
| Volume | 20 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sept 2023 |
Bibliographical note
Publisher Copyright:© 2023 Wiley-VHCA AG, Zurich, Switzerland.
Keywords
- Cladosporium oxysporum
- PPAR-γ
- acyclic diterpenes
- glucose uptake
- lipid accumulation