Phenethyl isothiocyanate decreases thymic stromal lymphopoietin-induced inflammatory reactions in mast cells

Na Ra Han, Phil Dong Moon, Ka Jung Ryu, Hyung Min Kim, Hyun Ja Jeong

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Phenethyl isothiocyanate (PEITC) has been reported to have anti-inflammatory and anti-carcinogenic properties. However, the anti-inflammatory effects of PEITC on thymic stromal lymphopoietin (TSLP)-induced inflammatory responses are uncertain. This study evaluates pharmacological activities of PEITC on inflammatory reactions in TSLP-stimulated mast cells. Human mast cell line HMC-1 was treated with PEITC (0.04, 0.4, and 4 µM) and subjected to inflammation by TSLP. Our results showed that PEITC significantly attenuated IL-13 and TNF-α levels increased by TSLP in HMC-1. PEITC significantly decreased TSLP-promoted HMC-1 proliferation and Ki67 mRNA expression. Protein levels of MDM2 and pSTAT6 increased by TSLP were significantly suppressed by PEITC in HMC-1. In addition, PEITC significantly enhanced protein levels of cleaved poly ADP-ribose polymerase and p53 decreased by TSLP. Based on the effects of PEITC on inflammation and proliferation in this study, it is possible that PEITC is a potential candidate to treat mast cells-mediated inflammatory disorders. Practical applications: This report provides strong evidence that Phenethyl isothiocyanate (PEITC) which is a dietary constituent derived from cruciferous vegetables, may be considered an alternative agent for treatment of mast cells-mediated inflammatory disorders.

Original languageEnglish
Article numbere12449
JournalJournal of Food Biochemistry
Volume42
Issue number1
DOIs
Publication statusPublished - 1 Feb 2018

Keywords

  • IL-13
  • inflammation
  • mast cells
  • phenethyl isothiocyanate
  • thymic stromal lymphopoietin

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