Phorbol ester or epidermal growth-factor-induced MUC5AC mucin gene expression and production from airway epithelial cells are inhibited by apigenin and wogonin

Ju Ock Kim, Md Asaduzzaman Sikder, Hyun Jae Lee, Mustafizur Rahman, Jang Hyun Kim, Gyu Tae Chang, Choong Jae Lee

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

In this study, we investigated whether apigenin and wogonin affect MUC5AC mucin production and gene expression induced by phorbol ester (phorbol 12-myristate 13-acetate, PMA) or epidermal growth factor (EGF) from human airway epithelial cells. Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with PMA or EGF for 24 h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA). The results were as follows: (i) apigenin and wogonin were found to inhibit the production of MUC5AC mucin protein induced by PMA or EGF; (ii) both compounds also inhibited the expression of MUC5AC mucin gene induced by PMA or EGF. These results suggest that apigenin and wogonin can inhibit mucin gene expression and production of mucin protein, by directly acting on airway epithelial cells.

Original languageEnglish
Pages (from-to)1784-1788
Number of pages5
JournalPhytotherapy Research
Volume26
Issue number12
DOIs
Publication statusPublished - Dec 2012

Keywords

  • MUC5AC
  • airway mucin
  • apigenin
  • wogonin

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