Photooxidation of RPE lipofuscin bisretinoids enhances fluorescence intensity

So Ra Kim; Young Pyo Jang; Janet R. Sparrow

doi:10.1016/j.visres.2009.09.015

Photooxidation of RPE lipofuscin bisretinoids enhances fluorescence intensity

So Ra Kim, Young Pyo Jang, Janet R. Sparrow

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Light-related cycling of chemically reactive vitamin A aldehyde leads to the formation autofluorescent bis-retinoid pigments that accumulate as lipofuscin in retinal pigment epithelial cells. The amassing of these diretinoid compounds is implicated in the pathogenesis of age-related macular degeneration and in some inherited forms of retinal degeneration. For all of these fluorophores, extended conjugation systems confer absorbance maxima in the visible spectrum. We report that an increase in fluorescence emission can accompany photoxidation of the bisretinoids A2E and all-trans-retinal dimer. These findings are relevant to the quantification of RPE lipofuscin based on inference from fluorescence intensity.

Original language	English
Pages (from-to)	729-736
Number of pages	8
Journal	Vision Research
Volume	50
Issue number	7
DOIs	https://doi.org/10.1016/j.visres.2009.09.015
Publication status	Published - Mar 2010

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health Grants EY12951 to Janet R. Sparrow and EY004367 to David R. Williams; a gift from Dr. Gertrude Neumark Rothschild; and a grant from Research to Prevent Blindness to the Department of Ophthalmology. J.R.S. is the recipient of a Research to Prevent Blindness Senior Investigator Award.

Keywords

Bisretinoids
Fluorescence
Lipofuscin
Photooxidation
Retinal pigment epithelium

Access to Document

10.1016/j.visres.2009.09.015

Cite this

@article{2c1befca9ba94c348a339144d5e64c3b,

title = "Photooxidation of RPE lipofuscin bisretinoids enhances fluorescence intensity",

abstract = "Light-related cycling of chemically reactive vitamin A aldehyde leads to the formation autofluorescent bis-retinoid pigments that accumulate as lipofuscin in retinal pigment epithelial cells. The amassing of these diretinoid compounds is implicated in the pathogenesis of age-related macular degeneration and in some inherited forms of retinal degeneration. For all of these fluorophores, extended conjugation systems confer absorbance maxima in the visible spectrum. We report that an increase in fluorescence emission can accompany photoxidation of the bisretinoids A2E and all-trans-retinal dimer. These findings are relevant to the quantification of RPE lipofuscin based on inference from fluorescence intensity.",

keywords = "Bisretinoids, Fluorescence, Lipofuscin, Photooxidation, Retinal pigment epithelium",

author = "Kim, {So Ra} and Jang, {Young Pyo} and Sparrow, {Janet R.}",

note = "Funding Information: This work was supported by National Institutes of Health Grants EY12951 to Janet R. Sparrow and EY004367 to David R. Williams; a gift from Dr. Gertrude Neumark Rothschild; and a grant from Research to Prevent Blindness to the Department of Ophthalmology. J.R.S. is the recipient of a Research to Prevent Blindness Senior Investigator Award.",

year = "2010",

month = mar,

doi = "10.1016/j.visres.2009.09.015",

language = "English",

volume = "50",

pages = "729--736",

journal = "Vision Research",

issn = "0042-6989",

publisher = "Elsevier Ltd.",

number = "7",

}

TY - JOUR

T1 - Photooxidation of RPE lipofuscin bisretinoids enhances fluorescence intensity

AU - Kim, So Ra

AU - Jang, Young Pyo

AU - Sparrow, Janet R.

N1 - Funding Information: This work was supported by National Institutes of Health Grants EY12951 to Janet R. Sparrow and EY004367 to David R. Williams; a gift from Dr. Gertrude Neumark Rothschild; and a grant from Research to Prevent Blindness to the Department of Ophthalmology. J.R.S. is the recipient of a Research to Prevent Blindness Senior Investigator Award.

PY - 2010/3

Y1 - 2010/3

N2 - Light-related cycling of chemically reactive vitamin A aldehyde leads to the formation autofluorescent bis-retinoid pigments that accumulate as lipofuscin in retinal pigment epithelial cells. The amassing of these diretinoid compounds is implicated in the pathogenesis of age-related macular degeneration and in some inherited forms of retinal degeneration. For all of these fluorophores, extended conjugation systems confer absorbance maxima in the visible spectrum. We report that an increase in fluorescence emission can accompany photoxidation of the bisretinoids A2E and all-trans-retinal dimer. These findings are relevant to the quantification of RPE lipofuscin based on inference from fluorescence intensity.

AB - Light-related cycling of chemically reactive vitamin A aldehyde leads to the formation autofluorescent bis-retinoid pigments that accumulate as lipofuscin in retinal pigment epithelial cells. The amassing of these diretinoid compounds is implicated in the pathogenesis of age-related macular degeneration and in some inherited forms of retinal degeneration. For all of these fluorophores, extended conjugation systems confer absorbance maxima in the visible spectrum. We report that an increase in fluorescence emission can accompany photoxidation of the bisretinoids A2E and all-trans-retinal dimer. These findings are relevant to the quantification of RPE lipofuscin based on inference from fluorescence intensity.

KW - Bisretinoids

KW - Fluorescence

KW - Lipofuscin

KW - Photooxidation

KW - Retinal pigment epithelium

UR - http://www.scopus.com/inward/record.url?scp=77950519368&partnerID=8YFLogxK

U2 - 10.1016/j.visres.2009.09.015

DO - 10.1016/j.visres.2009.09.015

M3 - Article

C2 - 19800359

AN - SCOPUS:77950519368

SN - 0042-6989

VL - 50

SP - 729

EP - 736

JO - Vision Research

JF - Vision Research

IS - 7

ER -

Photooxidation of RPE lipofuscin bisretinoids enhances fluorescence intensity

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this