Poncirus trifoliata fruit induces apoptosis in human promyelocytic leukemia cells

Jin Mu Yi; Mi Sun Kim; Hyun Na Koo; Bong Keun Song; Young Hyun Yoo; Hyung Min Kim

doi:10.1016/j.cccn.2003.10.017

Poncirus trifoliata fruit induces apoptosis in human promyelocytic leukemia cells

Jin Mu Yi
, Mi Sun Kim
, Hyun Na Koo
, Bong Keun Song
, Young Hyun Yoo
, Hyung Min Kim

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

Background: Substances inducing apoptosis have shown efficacy in the treatment of cancers. Poncirus trifoliata (L.) Raf. (Rutaceae) fruits (PTF) has been used for the treatment of various cancers among Korean Oriental Medical doctors. Methods: PTF-induced cytotoxicity of human leukemia HL-60 cells was monitored by the MTT assay. The apoptosis was determined by (a) apoptotic morphology in microscopy; (b) DNA fragmentation in electrophoresis and FACS analysis; and (c) activation of caspase-3 and poly-ADP-ribose polymerase (PARP) cleavage assay. Results: The cytotoxic activity of PTF in HL-60 cells was increased in a concentration- and time-dependent manner. PTF caused the cell shrinkage, cell membrane blebbing, apoptotic body and DNA fragmentation. PTF-induced apoptosis is accompanied by the activation of caspase-3 and the specific proteolytic cleavage of PARP. However, PTF did not show cytotoxicity in normal peripheral blood mononuclear cells. Conclusions: Our novel finding provides evidence that PTF could be a candidate as an anti-leukemic agent through apoptosis of cancer cells.

Original language	English
Pages (from-to)	179-185
Number of pages	7
Journal	Clinica Chimica Acta
Volume	340
Issue number	1-2
DOIs	https://doi.org/10.1016/j.cccn.2003.10.017
Publication status	Published - Feb 2004

Bibliographical note

Funding Information:
This research was supported by a grant (#PF 2–13) from Plant Diversity Research Center of the 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government and partially by Wonkwang University in 2002.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
Caspase-3
HL-60
Poncirus trifoliata fruit

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10.1016/j.cccn.2003.10.017

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@article{b3da3a64fd3e4d8a89ec8f210ef9fbeb,

title = "Poncirus trifoliata fruit induces apoptosis in human promyelocytic leukemia cells",

abstract = "Background: Substances inducing apoptosis have shown efficacy in the treatment of cancers. Poncirus trifoliata (L.) Raf. (Rutaceae) fruits (PTF) has been used for the treatment of various cancers among Korean Oriental Medical doctors. Methods: PTF-induced cytotoxicity of human leukemia HL-60 cells was monitored by the MTT assay. The apoptosis was determined by (a) apoptotic morphology in microscopy; (b) DNA fragmentation in electrophoresis and FACS analysis; and (c) activation of caspase-3 and poly-ADP-ribose polymerase (PARP) cleavage assay. Results: The cytotoxic activity of PTF in HL-60 cells was increased in a concentration- and time-dependent manner. PTF caused the cell shrinkage, cell membrane blebbing, apoptotic body and DNA fragmentation. PTF-induced apoptosis is accompanied by the activation of caspase-3 and the specific proteolytic cleavage of PARP. However, PTF did not show cytotoxicity in normal peripheral blood mononuclear cells. Conclusions: Our novel finding provides evidence that PTF could be a candidate as an anti-leukemic agent through apoptosis of cancer cells.",

keywords = "Apoptosis, Caspase-3, HL-60, Poncirus trifoliata fruit",

author = "Yi, \{Jin Mu\} and Kim, \{Mi Sun\} and Koo, \{Hyun Na\} and Song, \{Bong Keun\} and Yoo, \{Young Hyun\} and Kim, \{Hyung Min\}",

note = "Funding Information: This research was supported by a grant (\#PF 2–13) from Plant Diversity Research Center of the 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government and partially by Wonkwang University in 2002.",

year = "2004",

month = feb,

doi = "10.1016/j.cccn.2003.10.017",

language = "English",

volume = "340",

pages = "179--185",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Poncirus trifoliata fruit induces apoptosis in human promyelocytic leukemia cells

AU - Yi, Jin Mu

AU - Kim, Mi Sun

AU - Koo, Hyun Na

AU - Song, Bong Keun

AU - Yoo, Young Hyun

AU - Kim, Hyung Min

N1 - Funding Information: This research was supported by a grant (#PF 2–13) from Plant Diversity Research Center of the 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government and partially by Wonkwang University in 2002.

PY - 2004/2

Y1 - 2004/2

N2 - Background: Substances inducing apoptosis have shown efficacy in the treatment of cancers. Poncirus trifoliata (L.) Raf. (Rutaceae) fruits (PTF) has been used for the treatment of various cancers among Korean Oriental Medical doctors. Methods: PTF-induced cytotoxicity of human leukemia HL-60 cells was monitored by the MTT assay. The apoptosis was determined by (a) apoptotic morphology in microscopy; (b) DNA fragmentation in electrophoresis and FACS analysis; and (c) activation of caspase-3 and poly-ADP-ribose polymerase (PARP) cleavage assay. Results: The cytotoxic activity of PTF in HL-60 cells was increased in a concentration- and time-dependent manner. PTF caused the cell shrinkage, cell membrane blebbing, apoptotic body and DNA fragmentation. PTF-induced apoptosis is accompanied by the activation of caspase-3 and the specific proteolytic cleavage of PARP. However, PTF did not show cytotoxicity in normal peripheral blood mononuclear cells. Conclusions: Our novel finding provides evidence that PTF could be a candidate as an anti-leukemic agent through apoptosis of cancer cells.

AB - Background: Substances inducing apoptosis have shown efficacy in the treatment of cancers. Poncirus trifoliata (L.) Raf. (Rutaceae) fruits (PTF) has been used for the treatment of various cancers among Korean Oriental Medical doctors. Methods: PTF-induced cytotoxicity of human leukemia HL-60 cells was monitored by the MTT assay. The apoptosis was determined by (a) apoptotic morphology in microscopy; (b) DNA fragmentation in electrophoresis and FACS analysis; and (c) activation of caspase-3 and poly-ADP-ribose polymerase (PARP) cleavage assay. Results: The cytotoxic activity of PTF in HL-60 cells was increased in a concentration- and time-dependent manner. PTF caused the cell shrinkage, cell membrane blebbing, apoptotic body and DNA fragmentation. PTF-induced apoptosis is accompanied by the activation of caspase-3 and the specific proteolytic cleavage of PARP. However, PTF did not show cytotoxicity in normal peripheral blood mononuclear cells. Conclusions: Our novel finding provides evidence that PTF could be a candidate as an anti-leukemic agent through apoptosis of cancer cells.

KW - Apoptosis

KW - Caspase-3

KW - HL-60

KW - Poncirus trifoliata fruit

UR - https://www.scopus.com/pages/publications/0842281429

U2 - 10.1016/j.cccn.2003.10.017

DO - 10.1016/j.cccn.2003.10.017

M3 - Article

C2 - 14734210

AN - SCOPUS:0842281429

SN - 0009-8981

VL - 340

SP - 179

EP - 185

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 1-2

ER -

Poncirus trifoliata fruit induces apoptosis in human promyelocytic leukemia cells

Abstract

Bibliographical note

UN SDGs

Keywords

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