TY - GEN
T1 - Prediction of recovery based on solubility parameter value of anti-solvent in drowning-out of proteins
AU - Hwang, J. H.
AU - Kim, J. M.
AU - Chang, S. M.
AU - Kim, I. H.
AU - Kim, W. S.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006
Y1 - 2006
N2 - It requires that an anti-solvent is designed in relation to its precipitation efficacy in drowning-out process of proteins, more delicately. Based on the solubility parameter value of a specific solvent, we designed the forecasting model on protein precipitation in the present study. First, each protein recovery on drowning-out with some kinds of solvents was estimated by Miller-modified Lowry assay. And then the recovery data were plotted versus each solubility parameter value of the used solvents. Next, we obtained the model curve for prediction of recovery through regression and correlation analyses. Finally, to support our prediction on protein recovery due to a specific anti-solvent, the newly-designed anti-solvents were employed in the same procedure. The predicted values and the experimental results were compared. In this study, it was shown that protein recovery by drowning-out was closely correlated with solubility parameter value of a specific anti-solvent. Therefore, protein recovery may be forecasted with the selection of a specific second solvent in drowning-out, according to our semi-empirical recovery model.
AB - It requires that an anti-solvent is designed in relation to its precipitation efficacy in drowning-out process of proteins, more delicately. Based on the solubility parameter value of a specific solvent, we designed the forecasting model on protein precipitation in the present study. First, each protein recovery on drowning-out with some kinds of solvents was estimated by Miller-modified Lowry assay. And then the recovery data were plotted versus each solubility parameter value of the used solvents. Next, we obtained the model curve for prediction of recovery through regression and correlation analyses. Finally, to support our prediction on protein recovery due to a specific anti-solvent, the newly-designed anti-solvents were employed in the same procedure. The predicted values and the experimental results were compared. In this study, it was shown that protein recovery by drowning-out was closely correlated with solubility parameter value of a specific anti-solvent. Therefore, protein recovery may be forecasted with the selection of a specific second solvent in drowning-out, according to our semi-empirical recovery model.
KW - Drowning-out
KW - Protein recovery
KW - Semi-empirical prediction
KW - Solubility parameter
UR - http://www.scopus.com/inward/record.url?scp=34748813757&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:34748813757
SN - 8086059456
SN - 9788086059457
T3 - CHISA 2006 - 17th International Congress of Chemical and Process Engineering
BT - CHISA 2006 - 17th International Congress of Chemical and Process Engineering
T2 - CHISA 2006 - 17th International Congress of Chemical and Process Engineering
Y2 - 27 August 2006 through 31 August 2006
ER -