Preventive effects of bee venom derived phospholipase A2 on oxaliplatin-induced neuropathic pain in mice

Dongxing Li, Woojin Kim, Dasom Shin, Yongjae Jung, Hyunsu Bae, Sun Kwang Kim

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Oxaliplatin, a chemotherapy drug used to treat colorectal cancer, induces specific sensory neurotoxicity signs that are aggravated by cold and mechanical stimuli. Here we examined the preventive effects of Bee Venom (BV) derived phospholipase A2 (bvPLA2) on oxaliplatin-induced neuropathic pain in mice and its immunological mechanism. The cold and mechanical allodynia signs were evaluated by acetone and von Frey hair test on the hind paw, respectively. The most significant allodynia signs were observed at three days after an injection of oxaliplatin (6 mg/kg, i.p.) and then decreased gradually to a normal level on days 7–9. The oxaliplatin injection also induced infiltration of macrophages and upregulated levels of the pro-inflammatory cytokine interleukin (IL)-1β in the lumbar dorsal root ganglia (DRG). Daily treatment with bvPLA2 (0.2 mg/kg, i.p.) for five consecutive days prior to the oxaliplatin injection markedly inhibited the development of cold and mechanical allodynia, and suppressed infiltration of macrophages and the increase of IL-1β level in the DRG. Such preventive effects of bvPLA2 were completely blocked by depleting regulatory T cells (Tregs) with CD25 antibody pre-treatments. These results suggest that bvPLA2 may prevent oxaliplatin-induced neuropathic pain by suppressing immune responses in the DRG by Tregs.

Original languageEnglish
Article number27
JournalToxins
Volume8
Issue number1
DOIs
Publication statusPublished - 19 Jan 2016

Bibliographical note

Publisher Copyright:
© 2016 by the authors; licensee MDPI, Basel, Switzerland.

Keywords

  • Bee venom derived phospholipase A
  • Dorsal root ganglia
  • Neuropathic pain
  • Oxaliplatin
  • Regulatory T cells

Fingerprint

Dive into the research topics of 'Preventive effects of bee venom derived phospholipase A2 on oxaliplatin-induced neuropathic pain in mice'. Together they form a unique fingerprint.

Cite this