Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC

Mi Hyeon Jang; Min Chul Shin; Youn Jung Kim; Joo Ho Chung; Sung Vin Yim; Ee Hwa Kim; Yeunhee Kim; Chang Ju Kim

doi:10.1254/jjp.87.338

Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC

Mi Hyeon Jang, Min Chul Shin, Youn Jung Kim, Joo Ho Chung, Sung Vin Yim, Ee Hwa Kim, Yeunhee Kim, Chang Ju Kim

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18 Citations (Scopus)

Abstract

Puerariae flos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.

Original language	English
Pages (from-to)	338-342
Number of pages	5
Journal	Japanese Journal of Pharmacology
Volume	87
Issue number	4
DOIs	https://doi.org/10.1254/jjp.87.338
Publication status	Published - 2001

Keywords

Apoptosis
Ethanol
Puerariae flos

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10.1254/jjp.87.338

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title = "Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC",

abstract = "Puerariae flos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.",

keywords = "Apoptosis, Ethanol, Puerariae flos",

author = "Jang, {Mi Hyeon} and Shin, {Min Chul} and Kim, {Youn Jung} and Chung, {Joo Ho} and Yim, {Sung Vin} and Kim, {Ee Hwa} and Yeunhee Kim and Kim, {Chang Ju}",

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doi = "10.1254/jjp.87.338",

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T1 - Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC

AU - Jang, Mi Hyeon

AU - Shin, Min Chul

AU - Kim, Youn Jung

AU - Chung, Joo Ho

AU - Yim, Sung Vin

AU - Kim, Ee Hwa

AU - Kim, Yeunhee

AU - Kim, Chang Ju

PY - 2001

Y1 - 2001

N2 - Puerariae flos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.

AB - Puerariae flos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.

KW - Apoptosis

KW - Ethanol

KW - Puerariae flos

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U2 - 10.1254/jjp.87.338

DO - 10.1254/jjp.87.338

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AN - SCOPUS:0035544035

SN - 0021-5198

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EP - 342

JO - Japanese Journal of Pharmacology

JF - Japanese Journal of Pharmacology

IS - 4

ER -

Protective effects of Puerariae flos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC

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Keywords

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