Abstract
To develop novel markers for hepatocellular carcinomas (HCC), 10 cases were analyzed by 2-dimensional electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry. Results were compared to those of paired adjacent nontumorous liver tissues. Comparative analysis of the respective spot patterns in 2DE revealed that 3 variants of class 3 aldehyde dehydrogenase (ALDH-3) newly appeared while 2 variants of ALDH-2 diminished to undetectable levels in HCC. However, 4 ALDH-1 variants with different pls remained unaffected. This pattern of concomitant appearance and disappearance of ALDH-3 and ALDH-2 variants was consistently observed in all HCC tissues. Our results suggest that alterations of ALDH isozyme variants may be closely correlated to HCC and that proteomic analysis of these proteins might be a novel approach to identify the molecular events in detail during hepatocarcinogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 261-265 |
| Number of pages | 5 |
| Journal | International Journal of Cancer |
| Volume | 97 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 10 Jan 2002 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- 2-Dimensional gel electrophoresis
- Aldehyde dehydrogenase
- Hepatocellular carcinoma
- Matrix-assisted laser desorption ionization mass spectrometry
- Proteome
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