Quantification of marker compounds in Cirsium setidens Nakai by HPLC-DAD

Hyun Cheol Jeong; You Shin Shim; Young Kyoung Rhee; Sang Yoon Choi; Hee Do Hong; Jayong Chung; Myung Joo Han; Chang Won Cho

doi:10.1007/s10068-013-0241-3

Quantification of marker compounds in Cirsium setidens Nakai by HPLC-DAD

Hyun Cheol Jeong, You Shin Shim, Young Kyoung Rhee, Sang Yoon Choi, Hee Do Hong, Jayong Chung, Myung Joo Han, Chang Won Cho

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

An effective method of high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the simultaneous quantification of two marker compounds, pectolinarin and pectolinarigenin, in Cirsium setidens Nakai, a Korean wild herb. The two marker compounds of C. setidens were separated on a C18 column with a gradient system. The calibration curves of the two standard compounds exhibited good linearity (R ² >0.9992). The limits of detection (LOD) values of pectolinarin and pectolinarigenin were 0.52 and 0.41 μg/mL, respectively, while their limits of quantification (LOQ) values were 1.57 and 1.25 μg/mL, respectively. The relative standard deviations (RSDs) of the data of the intra- and inter-day experiments were less than 1.65 and 2.78%, respectively. The results of the recovery test were 98.06-105.81% with an RSD value of 1.87-3.50%. In conclusion, the results of validation showed that the proposed HPLC method was useful for the quantification of the two marker compounds in C. setidens.

Original language	English
Pages (from-to)	1481-1486
Number of pages	6
Journal	Food Science and Biotechnology
Volume	22
Issue number	6
DOIs	https://doi.org/10.1007/s10068-013-0241-3
Publication status	Published - Dec 2013

Bibliographical note

Funding Information:
Acknowledgments This research was supported by grants from the Globalization of Korean Foods R&D program, funded by the Ministry of Food, Agriculture, Forestry and Fisheries, Republic of Korea (No. 911027-1) and Korea Food Research Institute.

Keywords

Cirsium setidens Nakai
HPLC-DAD
flavone
marker compound
validation

Access to Document

10.1007/s10068-013-0241-3

Cite this

@article{482fb1b7c35d4242a9a72353349b7ad5,

title = "Quantification of marker compounds in Cirsium setidens Nakai by HPLC-DAD",

abstract = "An effective method of high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the simultaneous quantification of two marker compounds, pectolinarin and pectolinarigenin, in Cirsium setidens Nakai, a Korean wild herb. The two marker compounds of C. setidens were separated on a C18 column with a gradient system. The calibration curves of the two standard compounds exhibited good linearity (R 2 >0.9992). The limits of detection (LOD) values of pectolinarin and pectolinarigenin were 0.52 and 0.41 μg/mL, respectively, while their limits of quantification (LOQ) values were 1.57 and 1.25 μg/mL, respectively. The relative standard deviations (RSDs) of the data of the intra- and inter-day experiments were less than 1.65 and 2.78%, respectively. The results of the recovery test were 98.06-105.81% with an RSD value of 1.87-3.50%. In conclusion, the results of validation showed that the proposed HPLC method was useful for the quantification of the two marker compounds in C. setidens.",

keywords = "Cirsium setidens Nakai, HPLC-DAD, flavone, marker compound, validation",

author = "Jeong, {Hyun Cheol} and Shim, {You Shin} and Rhee, {Young Kyoung} and Choi, {Sang Yoon} and Hong, {Hee Do} and Jayong Chung and Han, {Myung Joo} and Cho, {Chang Won}",

note = "Funding Information: Acknowledgments This research was supported by grants from the Globalization of Korean Foods R&D program, funded by the Ministry of Food, Agriculture, Forestry and Fisheries, Republic of Korea (No. 911027-1) and Korea Food Research Institute.",

year = "2013",

month = dec,

doi = "10.1007/s10068-013-0241-3",

language = "English",

volume = "22",

pages = "1481--1486",

journal = "Food Science and Biotechnology",

issn = "1226-7708",

publisher = "The Korean Society of Food Science and Technology",

number = "6",

}

TY - JOUR

T1 - Quantification of marker compounds in Cirsium setidens Nakai by HPLC-DAD

AU - Jeong, Hyun Cheol

AU - Shim, You Shin

AU - Rhee, Young Kyoung

AU - Choi, Sang Yoon

AU - Hong, Hee Do

AU - Chung, Jayong

AU - Han, Myung Joo

AU - Cho, Chang Won

N1 - Funding Information: Acknowledgments This research was supported by grants from the Globalization of Korean Foods R&D program, funded by the Ministry of Food, Agriculture, Forestry and Fisheries, Republic of Korea (No. 911027-1) and Korea Food Research Institute.

PY - 2013/12

Y1 - 2013/12

N2 - An effective method of high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the simultaneous quantification of two marker compounds, pectolinarin and pectolinarigenin, in Cirsium setidens Nakai, a Korean wild herb. The two marker compounds of C. setidens were separated on a C18 column with a gradient system. The calibration curves of the two standard compounds exhibited good linearity (R 2 >0.9992). The limits of detection (LOD) values of pectolinarin and pectolinarigenin were 0.52 and 0.41 μg/mL, respectively, while their limits of quantification (LOQ) values were 1.57 and 1.25 μg/mL, respectively. The relative standard deviations (RSDs) of the data of the intra- and inter-day experiments were less than 1.65 and 2.78%, respectively. The results of the recovery test were 98.06-105.81% with an RSD value of 1.87-3.50%. In conclusion, the results of validation showed that the proposed HPLC method was useful for the quantification of the two marker compounds in C. setidens.

AB - An effective method of high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the simultaneous quantification of two marker compounds, pectolinarin and pectolinarigenin, in Cirsium setidens Nakai, a Korean wild herb. The two marker compounds of C. setidens were separated on a C18 column with a gradient system. The calibration curves of the two standard compounds exhibited good linearity (R 2 >0.9992). The limits of detection (LOD) values of pectolinarin and pectolinarigenin were 0.52 and 0.41 μg/mL, respectively, while their limits of quantification (LOQ) values were 1.57 and 1.25 μg/mL, respectively. The relative standard deviations (RSDs) of the data of the intra- and inter-day experiments were less than 1.65 and 2.78%, respectively. The results of the recovery test were 98.06-105.81% with an RSD value of 1.87-3.50%. In conclusion, the results of validation showed that the proposed HPLC method was useful for the quantification of the two marker compounds in C. setidens.

KW - Cirsium setidens Nakai

KW - HPLC-DAD

KW - flavone

KW - marker compound

KW - validation

UR - http://www.scopus.com/inward/record.url?scp=84890962767&partnerID=8YFLogxK

U2 - 10.1007/s10068-013-0241-3

DO - 10.1007/s10068-013-0241-3

M3 - Article

AN - SCOPUS:84890962767

SN - 1226-7708

VL - 22

SP - 1481

EP - 1486

JO - Food Science and Biotechnology

JF - Food Science and Biotechnology

IS - 6

ER -

Quantification of marker compounds in Cirsium setidens Nakai by HPLC-DAD

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this