Rapid Determination of Ginkgolic Acids in Ginkgo biloba Leaf Using Online Column Switching High-Performance Liquid Chromatography-Diode Array Detection and Confirmation by Liquid Chromatography-tandem Mass Spectrometry

Hyounyoung Lee, Heungyoul Lim, Juhong Yang, Jongki Hong

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

In this study, an improved method for the quantitative analysis of ginkgolic acids (GAs) in Ginkgo biloba leaf extract was developed. The samples were extracted with a mixture of chloroform and 50 % ethanol, after which the chloroform extract was dried and reconstituted in methanol. GAs with 13:0, 15:1, and 17:1 in the extract were successfully separated within 40 min and determined with high throughput performance using an online column-switching HPLC method using an SP column C8 SG80 (4.6 × 150 mm, 5 μm) and a Cadenza 5CD C18 column (4.6 × 150 mm, 3 μm). The developed HPLC method was validated for Ginkgo biloba leaf extract. The validation parameters were specificity, linearity, precision, accuracy, and limits of detection and quantitation (LODs and LOQs, respectively). It was found that all of the calibration curves showed good linearity (r2 > 0.9993) within the tested ranges. The LODs and LOQs were all lower than 0.04 μg/mL. The established method was found to be simple, rapid, and high throughput for the quantitative analysis of GAs in ten commercial Ginkgo biloba leaf extract and dietary supplements. The samples were also analyzed in LC-electrospray ionization (ESI) tandem mass spectrometry (MS/MS) - multiple-ion reaction monitoring (MRM) mode to confirm the identification results that were obtained by the column switching HPLC-DAD method. The developed method is considered to be suitable for the routine quality control and safety assurance of Ginkgo biloba leaf extract.

Original languageEnglish
Pages (from-to)3629-3634
Number of pages6
JournalBulletin of the Korean Chemical Society
Volume34
Issue number12
DOIs
Publication statusPublished - 20 Dec 2013

Keywords

  • Column switching HPLC-DAD
  • Ginkgo biloba
  • Ginkgolic acid
  • LC-MS/MS
  • Method validation

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