Real-time detection of neurotransmitter using enzyme-immobilized CNT network transistors in 11 vessel occlusion rat model

G. J. Lee, J. E. Lim, J. H. Park, Y. H. Eo, H. J. Park, S. K. Choi, S. Hong, H. K. Park

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Glutamate is the principal excitatory neurotransmitter in the brain, and its excessive release plays a key role in neuronal death associated with a wide range of neuronal disorders. We immobilized glutamate oxidase (GLOD) on carbon nanotube (CNT) network junctions by non-covalent functionalized method to detect L-glutamate. After immobilizing GLOD on CNT network, the excess reactive groups of linker molecule remaining on the CNT surface was deactivated and blocked by ethanolamine. The real time electronic response of CNT network transistors immobilized by GLOD was conducted with glutamate standard solution in vitro. For real-time in-vivo glutamate measurements, we prepared the 11 vessel occlusion rat model. The ultrahigh sensitivity, selectivity, and fast response time of GLOD-immobilized CNT network transistor could provide great potential for real time electronic detection in the extracellular glutamate level of the brain.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
Pages646-648
Number of pages3
Publication statusPublished - 2008
Event2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 - Quebec City, QC, United States
Duration: 1 Jun 20085 Jun 2008

Publication series

NameTechnical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
Volume2

Conference

Conference2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008
Country/TerritoryUnited States
CityQuebec City, QC
Period1/06/085/06/08

Keywords

  • 11 vessel occlusion rat model
  • CNT network
  • Neurotransmitter
  • Real-time detection
  • Transistor

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