Real-time monitoring of extracellular glutamate release on repetitive ischemic injury in global ischemia model

G. J. Lee; S. K. Choi; S. W. Kang; S. Choi; J. H. Park; D. H. Park; Y. H. Park; K. S. Kim; H. K. Park

doi:10.1007/978-3-642-03885-3_231

Real-time monitoring of extracellular glutamate release on repetitive ischemic injury in global ischemia model

G. J. Lee, S. K. Choi, S. W. Kang, S. Choi, J. H. Park, D. H. Park, Y. H. Park, K. S. Kim, H. K. Park

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

During the operation, neurosurgeons usually perform the multiple temporary occlusions of parental artery which may induce the neuronal damage. It is generally thought that neuronal damage by cerebral ischemia is associated with extracellular concentrations of the excitatory amino acids. In this experiment, we measured the dynamics of extracellular glutamate release in 11 vessel occlusion (VO) model during repeated occlusion within short interval. Changes in cerebral blood flow were monitored by laser-Doppler flowmetry simultaneously with cortical glutamate level measured by amperometric biosensor. During ischemic episode, the maximum change of glutamate release was gradually decreased as 112.38 ± 26.21 μM in first period, 82.63 ± 18.50 μM in second period, and 48.58 ± 11.89 μM in third period. The time interval be tween the ischemia induction and the beginning of glutamate release was increased as 106.7 ± 10.89 (sec) at first attack, 139.11 ± 3.87 (sec) in second attack, 169.00 ± 14.56 (sec) in third ischemic period. From the results of real-time monitoring about glutamate release in 11 VO model during repetitive ischemic episode, it was demonstrated that repetitive ischemia induced less glutamate release from neuronal cell than single ischemia due to endogeneous protective mechanism which delayed glutamate release time in later ischemic injury.

Original language	English
Title of host publication	World Congress on Medical Physics and Biomedical Engineering
Subtitle of host publication	Diagnostic and Therapeutic Instrumentation, Clinical Engineering
Publisher	Springer Verlag
Pages	835-837
Number of pages	3
Edition	7
ISBN (Print)	9783642038846
DOIs	https://doi.org/10.1007/978-3-642-03885-3_231
Publication status	Published - 2009
Event	World Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering - Munich, Germany Duration: 7 Sept 2009 → 12 Sept 2009

Publication series

Name	IFMBE Proceedings
Number	7
Volume	25
ISSN (Print)	1680-0737

Conference

Conference	World Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering
Country/Territory	Germany
City	Munich
Period	7/09/09 → 12/09/09

Keywords

11VO model
Glutamate
Real-time monitoring
Repetitive ischemic injury

Access to Document

10.1007/978-3-642-03885-3_231

Cite this

Lee, G. J., Choi, S. K., Kang, S. W., Choi, S., Park, J. H., Park, D. H., Park, Y. H., Kim, K. S., & Park, H. K. (2009). Real-time monitoring of extracellular glutamate release on repetitive ischemic injury in global ischemia model. In World Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering (7 ed., pp. 835-837). (IFMBE Proceedings; Vol. 25, No. 7). Springer Verlag. https://doi.org/10.1007/978-3-642-03885-3_231

@inproceedings{1e3e0112cd0044eb9e33ada43bf968a9,

title = "Real-time monitoring of extracellular glutamate release on repetitive ischemic injury in global ischemia model",

abstract = "During the operation, neurosurgeons usually perform the multiple temporary occlusions of parental artery which may induce the neuronal damage. It is generally thought that neuronal damage by cerebral ischemia is associated with extracellular concentrations of the excitatory amino acids. In this experiment, we measured the dynamics of extracellular glutamate release in 11 vessel occlusion (VO) model during repeated occlusion within short interval. Changes in cerebral blood flow were monitored by laser-Doppler flowmetry simultaneously with cortical glutamate level measured by amperometric biosensor. During ischemic episode, the maximum change of glutamate release was gradually decreased as 112.38 ± 26.21 μM in first period, 82.63 ± 18.50 μM in second period, and 48.58 ± 11.89 μM in third period. The time interval be tween the ischemia induction and the beginning of glutamate release was increased as 106.7 ± 10.89 (sec) at first attack, 139.11 ± 3.87 (sec) in second attack, 169.00 ± 14.56 (sec) in third ischemic period. From the results of real-time monitoring about glutamate release in 11 VO model during repetitive ischemic episode, it was demonstrated that repetitive ischemia induced less glutamate release from neuronal cell than single ischemia due to endogeneous protective mechanism which delayed glutamate release time in later ischemic injury.",

keywords = "11VO model, Glutamate, Real-time monitoring, Repetitive ischemic injury",

author = "Lee, {G. J.} and Choi, {S. K.} and Kang, {S. W.} and S. Choi and Park, {J. H.} and Park, {D. H.} and Park, {Y. H.} and Kim, {K. S.} and Park, {H. K.}",

year = "2009",

doi = "10.1007/978-3-642-03885-3_231",

language = "English",

isbn = "9783642038846",

series = "IFMBE Proceedings",

publisher = "Springer Verlag",

number = "7",

pages = "835--837",

booktitle = "World Congress on Medical Physics and Biomedical Engineering",

address = "Germany",

edition = "7",

note = "World Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering ; Conference date: 07-09-2009 Through 12-09-2009",

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Lee, GJ , Choi, SK, Kang, SW, Choi, S, Park, JH, Park, DH, Park, YH, Kim, KS & Park, HK 2009, Real-time monitoring of extracellular glutamate release on repetitive ischemic injury in global ischemia model. in World Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering. 7 edn, IFMBE Proceedings, no. 7, vol. 25, Springer Verlag, pp. 835-837, World Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering, Munich, Germany, 7/09/09. https://doi.org/10.1007/978-3-642-03885-3_231

Real-time monitoring of extracellular glutamate release on repetitive ischemic injury in global ischemia model. / Lee, G. J.; Choi, S. K.; Kang, S. W. et al.
World Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering. 7. ed. Springer Verlag, 2009. p. 835-837 (IFMBE Proceedings; Vol. 25, No. 7).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Lee, G. J.

AU - Choi, S. K.

AU - Kang, S. W.

AU - Choi, S.

AU - Park, J. H.

AU - Park, D. H.

AU - Park, Y. H.

AU - Kim, K. S.

AU - Park, H. K.

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N2 - During the operation, neurosurgeons usually perform the multiple temporary occlusions of parental artery which may induce the neuronal damage. It is generally thought that neuronal damage by cerebral ischemia is associated with extracellular concentrations of the excitatory amino acids. In this experiment, we measured the dynamics of extracellular glutamate release in 11 vessel occlusion (VO) model during repeated occlusion within short interval. Changes in cerebral blood flow were monitored by laser-Doppler flowmetry simultaneously with cortical glutamate level measured by amperometric biosensor. During ischemic episode, the maximum change of glutamate release was gradually decreased as 112.38 ± 26.21 μM in first period, 82.63 ± 18.50 μM in second period, and 48.58 ± 11.89 μM in third period. The time interval be tween the ischemia induction and the beginning of glutamate release was increased as 106.7 ± 10.89 (sec) at first attack, 139.11 ± 3.87 (sec) in second attack, 169.00 ± 14.56 (sec) in third ischemic period. From the results of real-time monitoring about glutamate release in 11 VO model during repetitive ischemic episode, it was demonstrated that repetitive ischemia induced less glutamate release from neuronal cell than single ischemia due to endogeneous protective mechanism which delayed glutamate release time in later ischemic injury.

AB - During the operation, neurosurgeons usually perform the multiple temporary occlusions of parental artery which may induce the neuronal damage. It is generally thought that neuronal damage by cerebral ischemia is associated with extracellular concentrations of the excitatory amino acids. In this experiment, we measured the dynamics of extracellular glutamate release in 11 vessel occlusion (VO) model during repeated occlusion within short interval. Changes in cerebral blood flow were monitored by laser-Doppler flowmetry simultaneously with cortical glutamate level measured by amperometric biosensor. During ischemic episode, the maximum change of glutamate release was gradually decreased as 112.38 ± 26.21 μM in first period, 82.63 ± 18.50 μM in second period, and 48.58 ± 11.89 μM in third period. The time interval be tween the ischemia induction and the beginning of glutamate release was increased as 106.7 ± 10.89 (sec) at first attack, 139.11 ± 3.87 (sec) in second attack, 169.00 ± 14.56 (sec) in third ischemic period. From the results of real-time monitoring about glutamate release in 11 VO model during repetitive ischemic episode, it was demonstrated that repetitive ischemia induced less glutamate release from neuronal cell than single ischemia due to endogeneous protective mechanism which delayed glutamate release time in later ischemic injury.

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KW - Repetitive ischemic injury

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Lee GJ , Choi SK, Kang SW, Choi S, Park JH, Park DH et al. Real-time monitoring of extracellular glutamate release on repetitive ischemic injury in global ischemia model. In World Congress on Medical Physics and Biomedical Engineering: Diagnostic and Therapeutic Instrumentation, Clinical Engineering. 7 ed. Springer Verlag. 2009. p. 835-837. (IFMBE Proceedings; 7). doi: 10.1007/978-3-642-03885-3_231

Real-time monitoring of extracellular glutamate release on repetitive ischemic injury in global ischemia model

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