Recovery effect of lignans and fermented extracts from Forsythia koreana flowers on pancreatic islets damaged by alloxan in zebrafish (Danio rerio)

Yeong Geun Lee; Isabel Rodriguez; Youn Hee Nam; Jung Eun Gwag; Sang Ho Woo; Hyoung Geun Kim; Jung Hwan Ko; Bin Na Hong; Tong Ho Kang; Nam In Baek

doi:10.1186/s13765-019-0411-y

Recovery effect of lignans and fermented extracts from Forsythia koreana flowers on pancreatic islets damaged by alloxan in zebrafish (Danio rerio)

Yeong Geun Lee, Isabel Rodriguez, Youn Hee Nam, Jung Eun Gwag, Sang Ho Woo, Hyoung Geun Kim, Jung Hwan Ko, Bin Na Hong, Tong Ho Kang, Nam In Baek

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Repeated column separation yielded four enterolactone type lignans from Forsythia koreana flowers (FKF), whose chemical structures were identified using several spectral technics. FKF MeOH extract (FKFM) and four lignans significantly recovered aloxan induced pancreatic islet in zebrafish. Especially, aglycones, 1 and 3, exhibited relatively higher activity than the lignan glycosides, 2 and 4. Therefore, FKFM was fermented using a Microbacterium esteraromaticum, BGP1, to yield the fermented FKFM (FKFM-BGP1). FKFM and FKFM-BGP1 were extracted using n-butanol to give n-BuOH fraction of each, FKFM-nB and FKFM-BGP1-nB, respectively. FKFM-BGP1-nB showed higher activity than FKFM-nB, as well the content of the aglycones, 1 and 3, in FKFM-BGP1-nB, 2.42 ± 0.01% and 1.15 ± 0.01%, was revealed to be much higher than that in FKFM-nB, 0.01 ± 0.01% and 0.01 ± 0.01%, respectively. In conclusion, the lignan aglycones 1 and 3 as well FKFM-BGP1-nB from F. koreana flowers were proved to be potential anti-diabetic agents. Furthermore, we suggest that antidiabetic efficacy of FKFM-BGP1-nB might be related to lignan aglycones 1 and 3.

Original language	English
Article number	7
Journal	Applied Biological Chemistry
Volume	62
Issue number	1
DOIs	https://doi.org/10.1186/s13765-019-0411-y
Publication status	Published - 1 Dec 2019

Bibliographical note

Publisher Copyright:
© 2019, The Author(s).

Keywords

Arctigenin
Diabetes
Fermentation
Forsythia koreana
Matairesinol
Zebrafish

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/s13765-019-0411-y

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Lee, Y. G., Rodriguez, I., Nam, Y. H., Gwag, J. E., Woo, S. H., Kim, H. G., Ko, J. H., Hong, B. N., Kang, T. H., & Baek, N. I. (2019). Recovery effect of lignans and fermented extracts from Forsythia koreana flowers on pancreatic islets damaged by alloxan in zebrafish (Danio rerio). Applied Biological Chemistry, 62(1), Article 7. https://doi.org/10.1186/s13765-019-0411-y

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title = "Recovery effect of lignans and fermented extracts from Forsythia koreana flowers on pancreatic islets damaged by alloxan in zebrafish (Danio rerio)",

abstract = "Repeated column separation yielded four enterolactone type lignans from Forsythia koreana flowers (FKF), whose chemical structures were identified using several spectral technics. FKF MeOH extract (FKFM) and four lignans significantly recovered aloxan induced pancreatic islet in zebrafish. Especially, aglycones, 1 and 3, exhibited relatively higher activity than the lignan glycosides, 2 and 4. Therefore, FKFM was fermented using a Microbacterium esteraromaticum, BGP1, to yield the fermented FKFM (FKFM-BGP1). FKFM and FKFM-BGP1 were extracted using n-butanol to give n-BuOH fraction of each, FKFM-nB and FKFM-BGP1-nB, respectively. FKFM-BGP1-nB showed higher activity than FKFM-nB, as well the content of the aglycones, 1 and 3, in FKFM-BGP1-nB, 2.42 ± 0.01% and 1.15 ± 0.01%, was revealed to be much higher than that in FKFM-nB, 0.01 ± 0.01% and 0.01 ± 0.01%, respectively. In conclusion, the lignan aglycones 1 and 3 as well FKFM-BGP1-nB from F. koreana flowers were proved to be potential anti-diabetic agents. Furthermore, we suggest that antidiabetic efficacy of FKFM-BGP1-nB might be related to lignan aglycones 1 and 3.",

keywords = "Arctigenin, Diabetes, Fermentation, Forsythia koreana, Matairesinol, Zebrafish",

author = "Lee, {Yeong Geun} and Isabel Rodriguez and Nam, {Youn Hee} and Gwag, {Jung Eun} and Woo, {Sang Ho} and Kim, {Hyoung Geun} and Ko, {Jung Hwan} and Hong, {Bin Na} and Kang, {Tong Ho} and Baek, {Nam In}",

note = "Publisher Copyright: {\textcopyright} 2019, The Author(s).",

year = "2019",

month = dec,

day = "1",

doi = "10.1186/s13765-019-0411-y",

language = "English",

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T1 - Recovery effect of lignans and fermented extracts from Forsythia koreana flowers on pancreatic islets damaged by alloxan in zebrafish (Danio rerio)

AU - Lee, Yeong Geun

AU - Rodriguez, Isabel

AU - Nam, Youn Hee

AU - Gwag, Jung Eun

AU - Woo, Sang Ho

AU - Kim, Hyoung Geun

AU - Ko, Jung Hwan

AU - Hong, Bin Na

AU - Kang, Tong Ho

AU - Baek, Nam In

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Repeated column separation yielded four enterolactone type lignans from Forsythia koreana flowers (FKF), whose chemical structures were identified using several spectral technics. FKF MeOH extract (FKFM) and four lignans significantly recovered aloxan induced pancreatic islet in zebrafish. Especially, aglycones, 1 and 3, exhibited relatively higher activity than the lignan glycosides, 2 and 4. Therefore, FKFM was fermented using a Microbacterium esteraromaticum, BGP1, to yield the fermented FKFM (FKFM-BGP1). FKFM and FKFM-BGP1 were extracted using n-butanol to give n-BuOH fraction of each, FKFM-nB and FKFM-BGP1-nB, respectively. FKFM-BGP1-nB showed higher activity than FKFM-nB, as well the content of the aglycones, 1 and 3, in FKFM-BGP1-nB, 2.42 ± 0.01% and 1.15 ± 0.01%, was revealed to be much higher than that in FKFM-nB, 0.01 ± 0.01% and 0.01 ± 0.01%, respectively. In conclusion, the lignan aglycones 1 and 3 as well FKFM-BGP1-nB from F. koreana flowers were proved to be potential anti-diabetic agents. Furthermore, we suggest that antidiabetic efficacy of FKFM-BGP1-nB might be related to lignan aglycones 1 and 3.

AB - Repeated column separation yielded four enterolactone type lignans from Forsythia koreana flowers (FKF), whose chemical structures were identified using several spectral technics. FKF MeOH extract (FKFM) and four lignans significantly recovered aloxan induced pancreatic islet in zebrafish. Especially, aglycones, 1 and 3, exhibited relatively higher activity than the lignan glycosides, 2 and 4. Therefore, FKFM was fermented using a Microbacterium esteraromaticum, BGP1, to yield the fermented FKFM (FKFM-BGP1). FKFM and FKFM-BGP1 were extracted using n-butanol to give n-BuOH fraction of each, FKFM-nB and FKFM-BGP1-nB, respectively. FKFM-BGP1-nB showed higher activity than FKFM-nB, as well the content of the aglycones, 1 and 3, in FKFM-BGP1-nB, 2.42 ± 0.01% and 1.15 ± 0.01%, was revealed to be much higher than that in FKFM-nB, 0.01 ± 0.01% and 0.01 ± 0.01%, respectively. In conclusion, the lignan aglycones 1 and 3 as well FKFM-BGP1-nB from F. koreana flowers were proved to be potential anti-diabetic agents. Furthermore, we suggest that antidiabetic efficacy of FKFM-BGP1-nB might be related to lignan aglycones 1 and 3.

KW - Arctigenin

KW - Diabetes

KW - Fermentation

KW - Forsythia koreana

KW - Matairesinol

KW - Zebrafish

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U2 - 10.1186/s13765-019-0411-y

DO - 10.1186/s13765-019-0411-y

M3 - Article

AN - SCOPUS:85062219095

SN - 2468-0834

VL - 62

JO - Applied Biological Chemistry

JF - Applied Biological Chemistry

IS - 1

M1 - 7

ER -

Recovery effect of lignans and fermented extracts from Forsythia koreana flowers on pancreatic islets damaged by alloxan in zebrafish (Danio rerio)

Abstract

Bibliographical note

Keywords

UN SDGs

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