Relationship between environmental conditions, TRI5 gene expression and deoxynivalenol production in stored Lentinula edodes infected with Fusarium graminearum

This study made the first attempt to relate the production of deoxynivalenol (DON) to the expression of TRI5 gene in Fusarium graminearum as a function of interacting environmental factors (water activity (a_w) (0.95-0.98), temperature (20-30 °C) and incubation time (7 day-28 day)), so as to investigate its production mechanisms in Lentinula edodes. Changes in temperature, water activity and incubation time could significantly (P<0.01) affect DON production and TRI5 gene expression. The highest DON concentration (793.5±27.4 μg/kg) and TRI5 gene expression (2^−ΔΔCt=38.8±4.8) were observed when the cultures were incubated at 20 °C and 0.98 a_w for 21 days. Multi-regression analysis was performed and nonlinear models based on polynomial equations were established to uncover the individual effects of temperature, water activity and incubation time as well as their interactions on DON production and TRI5 gene expression. The established model was further used to develop contour maps to predict the DON production and TRI5 gene expression in relation to storage conditions in L. edodes. Highly significant positive correlation between DON production and fold TRI5 gene expression (R=0.5534, P<0.0001) was observed in this commodity. The production mechanisms of DON in L. edodes revealed in the present study will be beneficial for developing more effective targeted control strategies.