Rhodanobacter koreensis sp. Nov., a bacterium isolated from tomato rhizosphere

Kyung Hwa Won, Hina Singh, Hien T.T. Ngo, Heung Min Son, Moo Chang Kook, Ki Young Kim, Tae Hoo Yi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated THG-DD7T, was isolated from tomato plant rhizosphere soil. Strain THG-DD7T grew optimally at 25–30 °C, at pH 7.0–7.5 and in the presence of 0.5% (w/v) NaCl. According to the results of 16S rRNA gene sequence comparisons, strain THG-DD7T was most closely related to Rhodanobacter umsongensis GR24-2T (98.2%), Rhodanobacter panaciterrae LnR5-47T (98.0%), Rhodanobacter soli DCY45T (97.9%), Rhodanobacter terrae GP18-1T (97.9%) and Dyella ginsengisoli Gsoil 3046T (97.7%). The DNA G+C content was 65.2 mol%. In DNA–DNA hybridization, the DNA relatedness levels between strain THG-DD7T and its closest phylogenetically neighbours were below 40.0%. The predominant isoprenoid quinone was ubiquinone Q-8. The major polar lipids were diphosphatidylglycerol, phosphtidylethanolamine, phosphatidyl-Nmethylethanolamine and phosphatidylglycerol. The major fatty acids were iso-C15: 0, iso-C16: 0, iso-C17: 0, anteiso-C15: 0 and iso-C17: 1ω9c and/or C16: 0 10-methyl (summed feature 9). These data supported the affiliation of strain THG-DD7T to the genus Rhodanobacter. The results of physiological and biochemical tests enabled strain THG-DD7T to be differentiated genotypically and phenotypically from the species of the genus Rhodanobacter with validly published names. Therefore, the novel isolate represents a novel species, for which the name Rhodanobacter koreensis sp. nov. is proposed. The type strain is THG-DD7T (=KACC 17650T=JCM 19614T).

Original languageEnglish
Pages (from-to)1180-1185
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
DOIs
Publication statusPublished - 2015

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© 2015 IUMS.

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