Rhodanobacter koreensis sp. Nov., a bacterium isolated from tomato rhizosphere

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated THG-DD7^T, was isolated from tomato plant rhizosphere soil. Strain THG-DD7^T grew optimally at 25–30 °C, at pH 7.0–7.5 and in the presence of 0.5% (w/v) NaCl. According to the results of 16S rRNA gene sequence comparisons, strain THG-DD7^T was most closely related to Rhodanobacter umsongensis GR24-2^T (98.2%), Rhodanobacter panaciterrae LnR5-47^T (98.0%), Rhodanobacter soli DCY45^T (97.9%), Rhodanobacter terrae GP18-1^T (97.9%) and Dyella ginsengisoli Gsoil 3046^T (97.7%). The DNA G+C content was 65.2 mol%. In DNA–DNA hybridization, the DNA relatedness levels between strain THG-DD7^T and its closest phylogenetically neighbours were below 40.0%. The predominant isoprenoid quinone was ubiquinone Q-8. The major polar lipids were diphosphatidylglycerol, phosphtidylethanolamine, phosphatidyl-Nmethylethanolamine and phosphatidylglycerol. The major fatty acids were iso-C_{15: 0}, iso-C_{16: 0}, iso-C_{17: 0}, anteiso-C_{15: 0} and iso-C_{17: 1}ω9c and/or C_{16: 0} 10-methyl (summed feature 9). These data supported the affiliation of strain THG-DD7^T to the genus Rhodanobacter. The results of physiological and biochemical tests enabled strain THG-DD7^T to be differentiated genotypically and phenotypically from the species of the genus Rhodanobacter with validly published names. Therefore, the novel isolate represents a novel species, for which the name Rhodanobacter koreensis sp. nov. is proposed. The type strain is THG-DD7^T (=KACC 17650^T=JCM 19614^T).