ROS-Responsive activatable photosensitizing agent for imaging and photodynamic therapy of activated macrophages

Hyunjin Kim, Youngmi Kim, In Hoo Kim, Kyungtae Kim, Yongdoo Choi

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)

Abstract

The optical properties of macrophage-targeted theranostic nanoparticles (MacTNP) prepared from a Chlorin e6 (Ce6)-hyaluronic acid (HA) conjugate can be activated by reactive oxygen species (ROS) in macrophage cells. MacTNP are nonfluorescent and nonphototoxic in their native state. However, when treated with ROS, especially peroxynitrite, they become highly fluorescent and phototoxic. In vitro cell studies show that MacTNP emit near-infrared (NIR) fluorescence inside activated macrophages. The NIR fluorescence is quenched in the extracellular environment. MacTNP are nontoxic in macrophages up to a Ce6 concentration of 10 μM in the absence of light. However, MacTNP become phototoxic upon illumination in a light dose-dependent manner. In particular, significantly higher phototoxic effect is observed in the activated macrophage cells compared to human dermal fibroblasts and non-activated macrophages. The ROS-responsive MacTNP, with their high target-to-background ratio, may have a significant potential in selective NIR fluorescence imaging and in subsequent photodynamic therapy of atherosclerosis with minimum side effects.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalTheranostics
Volume4
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • Activatable
  • Macrophage
  • Photodynamic therapy.
  • Reactive oxygen species
  • Theranostics

Fingerprint

Dive into the research topics of 'ROS-Responsive activatable photosensitizing agent for imaging and photodynamic therapy of activated macrophages'. Together they form a unique fingerprint.

Cite this