Rotary RT-PCR microdevice for ultrafast genetic analysis

J. H. Jung, S. J. Choi, B. H. Park, T. S. Seo

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this study, we demonstrated a novel method of reverse transcriptase-polymerase chain reaction (RT-PCR) using a rotary system for detecting influenza A virus subtype H1N1. The Rotary Genetic Analyzer consists of three parts including a disposable plastic PCR microchip, thermal blocks for temperature control, and a stepper motor for precise rotating of the chip. The microchip is spun on the thermal blocks automatically by LabVIEW program to perform the target gene amplification by using viral RNA templates. The RT-PCR amplicons were successfully produced in 22 min which were confirmed in the capillary electrophoresis (CE) microdevice demonstrating the ultrafast H1N1 detection.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages308-310
Number of pages3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2 Oct 20116 Oct 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume1

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period2/10/116/10/11

Keywords

  • Influenza A virus
  • Reverse transcriptase-polymerase chain reaction
  • Rotary
  • Virus detection

Fingerprint

Dive into the research topics of 'Rotary RT-PCR microdevice for ultrafast genetic analysis'. Together they form a unique fingerprint.

Cite this