TY - GEN
T1 - RT-PCR microdevice integrated with a rosgene strip for colorimetric detection of influenza H1N1 virus
AU - Kim, Yong Tae
AU - Chen, Yuchao
AU - Choi, Jong Young
AU - Seo, Tae Seok
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - A polymerase chain reaction (PCR) reactor was incorporated with an immunochromatographic ROSGENE strip for colorimetric detection of Influenza H1N1 virus. A reverse transcriptase-PCR (RT-PCR) cocktail, which contains Texas Redlabeled primers, biotin-dUTP, and Influenza H1N1 virus, was filled in the PCR chamber, and the target gene was amplified by RT-PCR. The resultant amplicons which were attached with biotin moieties and Texas Red haptens were first conjugated with the gold nanoparticle labeled antibody, and then captured on the streptavidin coated on the ROSGENE strip. The target virus is simply confirmed by observing a red color in the test line of the strip.
AB - A polymerase chain reaction (PCR) reactor was incorporated with an immunochromatographic ROSGENE strip for colorimetric detection of Influenza H1N1 virus. A reverse transcriptase-PCR (RT-PCR) cocktail, which contains Texas Redlabeled primers, biotin-dUTP, and Influenza H1N1 virus, was filled in the PCR chamber, and the target gene was amplified by RT-PCR. The resultant amplicons which were attached with biotin moieties and Texas Red haptens were first conjugated with the gold nanoparticle labeled antibody, and then captured on the streptavidin coated on the ROSGENE strip. The target virus is simply confirmed by observing a red color in the test line of the strip.
KW - Immunochromatographic strip
KW - Influenza H1N1 virus
KW - Microdevice
KW - RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=84883755214&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883755214
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 999
EP - 1001
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -