@inproceedings{dd42dd46d4974224bbecb2ef27fa3e28,
title = "Sample-pretreatment of influenza a virus based on the microbead incorporated centrifugal microdevice",
abstract = "In this study, we report an advanced sample-pretreatment microdevice that can be applied for RNA purification from nasal swaps of influenza A virus with high speed, sensitivity and reproducibility. We utilized tetraethoxyorthosilicate (TEOS)-treated glass microbeads as an RNA capture matrix, and designed a capillary valve and a siphon channel for the controllable loading of the washing solution and the elution solution, respectively. The PCR cocktail was used as an elution solution to investigate the feasibility of further integration into the PCR chip, and the clinical samples were successfully analyzed. The whole process could be complete in 440 sec, and the RNA capture yield was∼81%.",
keywords = "Centrifugal microdevice, Influenza A virus, RNA, Sample pretreatment",
author = "Jung, {J. H.} and Park, {B. H.} and Oh, {S. J.} and Seo, {T. S.}",
note = "Copyright: Copyright 2014 Elsevier B.V., All rights reserved.; 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
year = "2013",
language = "English",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "1308--1310",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
}