TY - GEN
T1 - Sample stacking capillary electrophoretic microdevice for highly sensitive mini Y short tandem repeat genotyping
AU - Chen, Y.
AU - Choi, J. Y.
AU - Choi, S. J.
AU - Seo, T. S.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - A sample stacking capillary electrophoretic (CE) microdevice was developed to enhance the detection sensitivity of mini Y STR genotyping. The mini Y STR includes redesigned primer sequences to generate smaller-sized PCR ampli-cons to improve the PCR efficiency and the success rate for a low copy number and degraded DNA sample. We design an elongated double-T shaped CE microchannel, and the mini Y STR amplicons are filled in a 10 mm-long stacking channel and preconcentrated in a defined narrow region through an optimized sample stacking CE scheme. The peak intensities of the mini Y amplicons in the electropherogram reveal more than 10-fold higher fluorescence intensities compared with that of a conventional cross-injection CE. Such signal enhancement allows us to successfully analyze the Y STR typing with only 25 pg of male genomic DNA, with high background of female genomic DNA, and with highly degraded male genomic DNA.
AB - A sample stacking capillary electrophoretic (CE) microdevice was developed to enhance the detection sensitivity of mini Y STR genotyping. The mini Y STR includes redesigned primer sequences to generate smaller-sized PCR ampli-cons to improve the PCR efficiency and the success rate for a low copy number and degraded DNA sample. We design an elongated double-T shaped CE microchannel, and the mini Y STR amplicons are filled in a 10 mm-long stacking channel and preconcentrated in a defined narrow region through an optimized sample stacking CE scheme. The peak intensities of the mini Y amplicons in the electropherogram reveal more than 10-fold higher fluorescence intensities compared with that of a conventional cross-injection CE. Such signal enhancement allows us to successfully analyze the Y STR typing with only 25 pg of male genomic DNA, with high background of female genomic DNA, and with highly degraded male genomic DNA.
KW - Capillary electrophoresis
KW - Detection sensitivity
KW - Mini Y short tandem repeat
KW - Sample stacking
UR - http://www.scopus.com/inward/record.url?scp=84884307926&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884307926
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 1742
EP - 1744
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -