Selective Butyrate Esterase Probe for the Rapid Colorimetric and Fluorogenic Identification of Moraxella catarrhalis

Uisung Lee, Yeon Ho Kim, Ki Sun Yoon, Youngmi Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Clinical identification of the pathogenic bacterium Moraxella catarrhalis in cultures relies on the detection of bacterial butyrate esterase (C4-esterase) using a coumarin-based fluorogenic substrate, 4-methylumbelliferyl butyrate. However, this classical probe may give false-positive responses because of its poor stability and lack of specificity. Here, we report a new colorimetric and fluorogenic probe design employing a meso-ester-substituted boron dipyrromethene (BODIPY) dye for the specific detection of C4-esterase activity expressed by M. catarrhalis. This new probe has resistance to nonspecific hydrolysis that is far superior to the classical probe and also selectively responds to esterase with rapid colorimetric and fluorescence signal changes and large "turn-on"ratios. The probe was successfully applied to the specific detection of M. catarrhalis with high sensitivity.

Original languageEnglish
Pages (from-to)16051-16057
Number of pages7
JournalAnalytical Chemistry
Volume92
Issue number24
DOIs
Publication statusPublished - 15 Dec 2020

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