Selective Monitoring and Imaging of Eosinophil Peroxidase Activity with a J-Aggregating Probe

Tae Il Kim, Byunghee Hwang, Boeun Lee, Jeehyeon Bae, Youngmi Kim

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.

Original languageEnglish
Pages (from-to)11771-11776
Number of pages6
JournalJournal of the American Chemical Society
Volume140
Issue number37
DOIs
Publication statusPublished - 19 Sep 2018

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