Selective Monitoring and Imaging of Eosinophil Peroxidase Activity with a J-Aggregating Probe

Tae Il Kim; Byunghee Hwang; Boeun Lee; Jeehyeon Bae; Youngmi Kim

doi:10.1021/jacs.8b07073

Selective Monitoring and Imaging of Eosinophil Peroxidase Activity with a J-Aggregating Probe

Tae Il Kim, Byunghee Hwang, Boeun Lee, Jeehyeon Bae, Youngmi Kim

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.

Original language	English
Pages (from-to)	11771-11776
Number of pages	6
Journal	Journal of the American Chemical Society
Volume	140
Issue number	37
DOIs	https://doi.org/10.1021/jacs.8b07073
Publication status	Published - 19 Sept 2018

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Publisher Copyright:
© 2018 American Chemical Society.

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abstract = "The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.",

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AU - Kim, Tae Il

AU - Hwang, Byunghee

AU - Lee, Boeun

AU - Bae, Jeehyeon

AU - Kim, Youngmi

PY - 2018/9/19

Y1 - 2018/9/19

N2 - The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.

AB - The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.

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DO - 10.1021/jacs.8b07073

M3 - Article

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AN - SCOPUS:85053265701

SN - 0002-7863

VL - 140

SP - 11771

EP - 11776

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 37

ER -

Selective Monitoring and Imaging of Eosinophil Peroxidase Activity with a J-Aggregating Probe

Abstract

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