Shp2 is involved in neuronal differentiation of hippocampal precursor cells

Hak Jae Kim; Mi Han Ah; Hwan Shim Jae; Hyun Yoon Hye; Hyockman Kwon; Kim Kwon Yunhee

doi:10.1007/BF02977638

Shp2 is involved in neuronal differentiation of hippocampal precursor cells

Hak Jae Kim, Mi Han Ah, Hwan Shim Jae, Hyun Yoon Hye, Hyockman Kwon, Kim Kwon Yunhee

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6 Citations (Scopus)

Abstract

HiB5 is a multipotent hippocampal stem cell line whose differentiation into cells of a neuronal phenotype is promoted by neurotrophic factors such as PDGF and brain-derived neurotrophic factor (BDNF). We examined the potential role of Src homology 2 (SH2)-containing protein tyrosine phosphatase (Shp2) in this differentiation process. We found that Shp2 became tyrosine phosphorylated following PDGF treatment. Wild-type Shp2 enhanced the expression of neurofilament, synapsin I and PSD95 by PDGF and BDNF, whereas their expression was attenuated by the catalytically inactive mutants Shp2C/S and Shp2ΔP. Formation of dendritic spine-like structures increased with wild-type Shp2, but diminished with Shp2C/S and Shp2ΔP. PSD95, localized in the post-synaptic density region of dendritic spines, PDGFRβ and TrkB were co-immunoprecipitated with Shp2 antibodies. These results suggest that Shp2 plays a positive role in mediating PDGF- and BDNF-activated signaling which promotes the formation of dendritic spines.

Original language	English
Pages (from-to)	750-754
Number of pages	5
Journal	Archives of Pharmacal Research
Volume	30
Issue number	6
DOIs	https://doi.org/10.1007/BF02977638
Publication status	Published - 30 Jun 2007

Bibliographical note

Funding Information:
This work was supported by grants from the Brain Research Center (M103KV010007-06K2201-00710) of the 21C Frontier Research program by MOST, the Korea Health 21R&D Project (0405-DB01-0104-0006) by MOHW, and the BK21 by MOE to Y.K.K. and Ministry of Health & Welfare (O0-PJ9-PG1-CO05-0002) to H.K. We also thank the Korea Basic Science Institute for the support of laser scanning microscopy.

Keywords

BDNF
Differentiation
Neuron
PDGF
Shp2

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title = "Shp2 is involved in neuronal differentiation of hippocampal precursor cells",

abstract = "HiB5 is a multipotent hippocampal stem cell line whose differentiation into cells of a neuronal phenotype is promoted by neurotrophic factors such as PDGF and brain-derived neurotrophic factor (BDNF). We examined the potential role of Src homology 2 (SH2)-containing protein tyrosine phosphatase (Shp2) in this differentiation process. We found that Shp2 became tyrosine phosphorylated following PDGF treatment. Wild-type Shp2 enhanced the expression of neurofilament, synapsin I and PSD95 by PDGF and BDNF, whereas their expression was attenuated by the catalytically inactive mutants Shp2C/S and Shp2ΔP. Formation of dendritic spine-like structures increased with wild-type Shp2, but diminished with Shp2C/S and Shp2ΔP. PSD95, localized in the post-synaptic density region of dendritic spines, PDGFRβ and TrkB were co-immunoprecipitated with Shp2 antibodies. These results suggest that Shp2 plays a positive role in mediating PDGF- and BDNF-activated signaling which promotes the formation of dendritic spines.",

keywords = "BDNF, Differentiation, Neuron, PDGF, Shp2",

author = "Kim, {Hak Jae} and Ah, {Mi Han} and Jae, {Hwan Shim} and Hye, {Hyun Yoon} and Hyockman Kwon and Yunhee, {Kim Kwon}",

note = "Funding Information: This work was supported by grants from the Brain Research Center (M103KV010007-06K2201-00710) of the 21C Frontier Research program by MOST, the Korea Health 21R&D Project (0405-DB01-0104-0006) by MOHW, and the BK21 by MOE to Y.K.K. and Ministry of Health & Welfare (O0-PJ9-PG1-CO05-0002) to H.K. We also thank the Korea Basic Science Institute for the support of laser scanning microscopy.",

year = "2007",

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doi = "10.1007/BF02977638",

language = "English",

volume = "30",

pages = "750--754",

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T1 - Shp2 is involved in neuronal differentiation of hippocampal precursor cells

AU - Kim, Hak Jae

AU - Ah, Mi Han

AU - Jae, Hwan Shim

AU - Hye, Hyun Yoon

AU - Kwon, Hyockman

AU - Yunhee, Kim Kwon

N1 - Funding Information: This work was supported by grants from the Brain Research Center (M103KV010007-06K2201-00710) of the 21C Frontier Research program by MOST, the Korea Health 21R&D Project (0405-DB01-0104-0006) by MOHW, and the BK21 by MOE to Y.K.K. and Ministry of Health & Welfare (O0-PJ9-PG1-CO05-0002) to H.K. We also thank the Korea Basic Science Institute for the support of laser scanning microscopy.

PY - 2007/6/30

Y1 - 2007/6/30

N2 - HiB5 is a multipotent hippocampal stem cell line whose differentiation into cells of a neuronal phenotype is promoted by neurotrophic factors such as PDGF and brain-derived neurotrophic factor (BDNF). We examined the potential role of Src homology 2 (SH2)-containing protein tyrosine phosphatase (Shp2) in this differentiation process. We found that Shp2 became tyrosine phosphorylated following PDGF treatment. Wild-type Shp2 enhanced the expression of neurofilament, synapsin I and PSD95 by PDGF and BDNF, whereas their expression was attenuated by the catalytically inactive mutants Shp2C/S and Shp2ΔP. Formation of dendritic spine-like structures increased with wild-type Shp2, but diminished with Shp2C/S and Shp2ΔP. PSD95, localized in the post-synaptic density region of dendritic spines, PDGFRβ and TrkB were co-immunoprecipitated with Shp2 antibodies. These results suggest that Shp2 plays a positive role in mediating PDGF- and BDNF-activated signaling which promotes the formation of dendritic spines.

AB - HiB5 is a multipotent hippocampal stem cell line whose differentiation into cells of a neuronal phenotype is promoted by neurotrophic factors such as PDGF and brain-derived neurotrophic factor (BDNF). We examined the potential role of Src homology 2 (SH2)-containing protein tyrosine phosphatase (Shp2) in this differentiation process. We found that Shp2 became tyrosine phosphorylated following PDGF treatment. Wild-type Shp2 enhanced the expression of neurofilament, synapsin I and PSD95 by PDGF and BDNF, whereas their expression was attenuated by the catalytically inactive mutants Shp2C/S and Shp2ΔP. Formation of dendritic spine-like structures increased with wild-type Shp2, but diminished with Shp2C/S and Shp2ΔP. PSD95, localized in the post-synaptic density region of dendritic spines, PDGFRβ and TrkB were co-immunoprecipitated with Shp2 antibodies. These results suggest that Shp2 plays a positive role in mediating PDGF- and BDNF-activated signaling which promotes the formation of dendritic spines.

KW - BDNF

KW - Differentiation

KW - Neuron

KW - PDGF

KW - Shp2

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U2 - 10.1007/BF02977638

DO - 10.1007/BF02977638

M3 - Article

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AN - SCOPUS:34347393148

SN - 0253-6269

VL - 30

SP - 750

EP - 754

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

IS - 6

ER -

Shp2 is involved in neuronal differentiation of hippocampal precursor cells

Abstract

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Keywords

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