Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLC-PAD

Sa Im Lee; Ha Jeong Kwon; Yong Moon Lee; Je Hyun Lee; Seon Pyo Hong

doi:10.1016/j.jpba.2011.08.030

Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLC-PAD

Sa Im Lee, Ha Jeong Kwon, Yong Moon Lee, Je Hyun Lee, Seon Pyo Hong

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The paper describes the development of a simultaneous determination method for polar and non-polar ginsenosides in red ginseng with a reversed-phase high-performance liquid chromatography-pulsed amperometric detection method. This method could be applied directly without any pretreatment steps and enabled the performance of highly sensitive analysis within 1. h. The detection (S/. N= 3) and quantification (S/. N= 10) limits for the ginsenosides ranged 0.02-0.10. ng and 0.1-0.3. ng, respectively. The linear regression coefficients ranged 0.9975-0.9998. Intra- and inter-day precisions were <9.91%. The mean recoveries ranged 98.08-103.06%. The total amount of ginsenosides in the hairy root of red ginseng was higher than that in the main root.

Original language	English
Pages (from-to)	80-85
Number of pages	6
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	60
DOIs	https://doi.org/10.1016/j.jpba.2011.08.030
Publication status	Published - 23 Feb 2012

Bibliographical note

Funding Information:
This study was supported by a Korea Research Foundation (KRF) grant funded by the Korea government (MEST) (No. 2011-0003260).

Keywords

Column temperature
Ginsenosides
Pulsed amperometric detection
Red ginseng
Simultaneous determination

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10.1016/j.jpba.2011.08.030

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title = "Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLC-PAD",

abstract = "The paper describes the development of a simultaneous determination method for polar and non-polar ginsenosides in red ginseng with a reversed-phase high-performance liquid chromatography-pulsed amperometric detection method. This method could be applied directly without any pretreatment steps and enabled the performance of highly sensitive analysis within 1. h. The detection (S/. N= 3) and quantification (S/. N= 10) limits for the ginsenosides ranged 0.02-0.10. ng and 0.1-0.3. ng, respectively. The linear regression coefficients ranged 0.9975-0.9998. Intra- and inter-day precisions were <9.91%. The mean recoveries ranged 98.08-103.06%. The total amount of ginsenosides in the hairy root of red ginseng was higher than that in the main root.",

keywords = "Column temperature, Ginsenosides, Pulsed amperometric detection, Red ginseng, Simultaneous determination",

author = "Lee, {Sa Im} and Kwon, {Ha Jeong} and Lee, {Yong Moon} and Lee, {Je Hyun} and Hong, {Seon Pyo}",

note = "Funding Information: This study was supported by a Korea Research Foundation (KRF) grant funded by the Korea government (MEST) (No. 2011-0003260).",

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doi = "10.1016/j.jpba.2011.08.030",

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AU - Lee, Sa Im

AU - Kwon, Ha Jeong

AU - Lee, Yong Moon

AU - Lee, Je Hyun

AU - Hong, Seon Pyo

N1 - Funding Information: This study was supported by a Korea Research Foundation (KRF) grant funded by the Korea government (MEST) (No. 2011-0003260).

PY - 2012/2/23

Y1 - 2012/2/23

N2 - The paper describes the development of a simultaneous determination method for polar and non-polar ginsenosides in red ginseng with a reversed-phase high-performance liquid chromatography-pulsed amperometric detection method. This method could be applied directly without any pretreatment steps and enabled the performance of highly sensitive analysis within 1. h. The detection (S/. N= 3) and quantification (S/. N= 10) limits for the ginsenosides ranged 0.02-0.10. ng and 0.1-0.3. ng, respectively. The linear regression coefficients ranged 0.9975-0.9998. Intra- and inter-day precisions were <9.91%. The mean recoveries ranged 98.08-103.06%. The total amount of ginsenosides in the hairy root of red ginseng was higher than that in the main root.

AB - The paper describes the development of a simultaneous determination method for polar and non-polar ginsenosides in red ginseng with a reversed-phase high-performance liquid chromatography-pulsed amperometric detection method. This method could be applied directly without any pretreatment steps and enabled the performance of highly sensitive analysis within 1. h. The detection (S/. N= 3) and quantification (S/. N= 10) limits for the ginsenosides ranged 0.02-0.10. ng and 0.1-0.3. ng, respectively. The linear regression coefficients ranged 0.9975-0.9998. Intra- and inter-day precisions were <9.91%. The mean recoveries ranged 98.08-103.06%. The total amount of ginsenosides in the hairy root of red ginseng was higher than that in the main root.

KW - Column temperature

KW - Ginsenosides

KW - Pulsed amperometric detection

KW - Red ginseng

KW - Simultaneous determination

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JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

ER -

Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLC-PAD

Abstract

Bibliographical note

Keywords

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