Simultaneous electromechanical monitoring in engineered heart tissues using a mesoscale framework

Dominic E. Fullenkamp; Woo Youl Maeng; Seyong Oh; Haiwen Luan; Kyung Su Kim; Ivana A. Chychula; Jin Tae Kim; Jae Young Yoo; Cory W. Holgren; Alexis R. Demonbreun; Sharon George; Binjie Li; Yaching Hsu; Gooyoon Chung; Jeongmin Yoo; Jahyun Koo; Yoonseok Park; Igor R. Efimov; Elizabeth M. McNally; John A. Rogers

doi:10.1126/sciadv.ado7089

Simultaneous electromechanical monitoring in engineered heart tissues using a mesoscale framework

Dominic E. Fullenkamp, Woo Youl Maeng, Seyong Oh, Haiwen Luan, Kyung Su Kim, Ivana A. Chychula, Jin Tae Kim, Jae Young Yoo, Cory W. Holgren, Alexis R. Demonbreun, Sharon George, Binjie Li, Yaching Hsu, Gooyoon Chung, Jeongmin Yoo, Jahyun Koo, Yoonseok Park, Igor R. Efimov, Elizabeth M. McNally, John A. Rogers

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3 Citations (Scopus)

Abstract

Engineered heart tissues (EHTs) generated from human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs) represent powerful platforms for human cardiac research, especially in drug testing and disease modeling. Here, we report a flexible, three-dimensional electronic framework that enables real-time, spatiotemporal analysis of electrophysiologic and mechanical signals in EHTs under physiological loading conditions for dynamic, noninvasive, longer-term assessments. These electromechanically monitored EHTs support multisite measurements throughout the tissue under baseline conditions and in response to stimuli. Demonstrations include uses in tracking physiological responses to pharmacologically active agents and in capturing electrophysiological characteristics of reentrant arrhythmias. This platform facilitates precise analysis of signal location and conduction velocity in human cardiomyocyte tissues, as the basis for a broad range of advanced cardiovascular studies.

Original language	English
Article number	eado7089
Journal	Science advances
Volume	10
Issue number	37
DOIs	https://doi.org/10.1126/sciadv.ado7089
Publication status	Published - 13 Sept 2024

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Publisher Copyright:
Copyright © 2024 The Authors

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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Fullenkamp, D. E., Maeng, W. Y., Oh, S., Luan, H., Kim, K. S., Chychula, I. A., Kim, J. T., Yoo, J. Y., Holgren, C. W., Demonbreun, A. R., George, S., Li, B., Hsu, Y., Chung, G., Yoo, J., Koo, J., Park, Y., Efimov, I. R., McNally, E. M., & Rogers, J. A. (2024). Simultaneous electromechanical monitoring in engineered heart tissues using a mesoscale framework. Science advances, 10(37), Article eado7089. https://doi.org/10.1126/sciadv.ado7089

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title = "Simultaneous electromechanical monitoring in engineered heart tissues using a mesoscale framework",

abstract = "Engineered heart tissues (EHTs) generated from human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs) represent powerful platforms for human cardiac research, especially in drug testing and disease modeling. Here, we report a flexible, three-dimensional electronic framework that enables real-time, spatiotemporal analysis of electrophysiologic and mechanical signals in EHTs under physiological loading conditions for dynamic, noninvasive, longer-term assessments. These electromechanically monitored EHTs support multisite measurements throughout the tissue under baseline conditions and in response to stimuli. Demonstrations include uses in tracking physiological responses to pharmacologically active agents and in capturing electrophysiological characteristics of reentrant arrhythmias. This platform facilitates precise analysis of signal location and conduction velocity in human cardiomyocyte tissues, as the basis for a broad range of advanced cardiovascular studies.",

author = "Fullenkamp, {Dominic E.} and Maeng, {Woo Youl} and Seyong Oh and Haiwen Luan and Kim, {Kyung Su} and Chychula, {Ivana A.} and Kim, {Jin Tae} and Yoo, {Jae Young} and Holgren, {Cory W.} and Demonbreun, {Alexis R.} and Sharon George and Binjie Li and Yaching Hsu and Gooyoon Chung and Jeongmin Yoo and Jahyun Koo and Yoonseok Park and Efimov, {Igor R.} and McNally, {Elizabeth M.} and Rogers, {John A.}",

note = "Publisher Copyright: Copyright {\textcopyright} 2024 The Authors",

year = "2024",

month = sep,

day = "13",

doi = "10.1126/sciadv.ado7089",

language = "English",

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Fullenkamp, DE, Maeng, WY, Oh, S, Luan, H, Kim, KS, Chychula, IA, Kim, JT, Yoo, JY, Holgren, CW, Demonbreun, AR, George, S, Li, B, Hsu, Y, Chung, G, Yoo, J, Koo, J, Park, Y, Efimov, IR, McNally, EM & Rogers, JA 2024, 'Simultaneous electromechanical monitoring in engineered heart tissues using a mesoscale framework', Science advances, vol. 10, no. 37, eado7089. https://doi.org/10.1126/sciadv.ado7089

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T1 - Simultaneous electromechanical monitoring in engineered heart tissues using a mesoscale framework

AU - Fullenkamp, Dominic E.

AU - Maeng, Woo Youl

AU - Oh, Seyong

AU - Luan, Haiwen

AU - Kim, Kyung Su

AU - Chychula, Ivana A.

AU - Kim, Jin Tae

AU - Yoo, Jae Young

AU - Holgren, Cory W.

AU - Demonbreun, Alexis R.

AU - George, Sharon

AU - Li, Binjie

AU - Hsu, Yaching

AU - Chung, Gooyoon

AU - Yoo, Jeongmin

AU - Koo, Jahyun

AU - Park, Yoonseok

AU - Efimov, Igor R.

AU - McNally, Elizabeth M.

AU - Rogers, John A.

PY - 2024/9/13

Y1 - 2024/9/13

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AB - Engineered heart tissues (EHTs) generated from human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CMs) represent powerful platforms for human cardiac research, especially in drug testing and disease modeling. Here, we report a flexible, three-dimensional electronic framework that enables real-time, spatiotemporal analysis of electrophysiologic and mechanical signals in EHTs under physiological loading conditions for dynamic, noninvasive, longer-term assessments. These electromechanically monitored EHTs support multisite measurements throughout the tissue under baseline conditions and in response to stimuli. Demonstrations include uses in tracking physiological responses to pharmacologically active agents and in capturing electrophysiological characteristics of reentrant arrhythmias. This platform facilitates precise analysis of signal location and conduction velocity in human cardiomyocyte tissues, as the basis for a broad range of advanced cardiovascular studies.

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DO - 10.1126/sciadv.ado7089

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SN - 2375-2548

VL - 10

JO - Science advances

JF - Science advances

IS - 37

M1 - eado7089

ER -

Simultaneous electromechanical monitoring in engineered heart tissues using a mesoscale framework

Abstract

Bibliographical note

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