@inproceedings{59c4c4afbc514026bf1eb27367be56d5,
title = "Simultaneous measurement of nitric oxide and oxygen dynamics during myocardial ischemia reperfusion of rat",
abstract = "Myocardial infarction is one of the major causes of morbidity and mortality in industrialized countries despite the improvement in clinical management of the disease. Myocardial function is dependent on a constant supply of oxygen from coronary circulation. Therefore the accurate measurement of oxygen concentration in postischemic myocardium is crucial to the assessment of myocardial viability and function. Nitric oxide (NO) plays important roles in the cardiovascular system by mediating various physiological and pathophysiological processes. However, the role of endogenous NO in ischemia-reperfusion is still controversial. Besides, direct and real-time monitoring of NO is difficult because NO is present only at low concentrations in the body and has a short half-life Therefore, we utilized a sol-gel derived electrochemical NO and oxygen microsensor to monitor changes in NO and oxygen during myocardial ischemia-reperfusion. NO and oxygen sensors were built using a xerogel-modified platinum microsensor and coiled Ag/AgCl reference electrode. Hearts were placed on a Langendorff apparatus and treated by 30 min of ischemia and 60 min of reperfusion. In the ischemic myocardium, NO showed a time-course dependent change during the 30 min ischemic episode. And the restoration concentration of NO and oxygen was decreased below the pre-ischemic level after myocardial ischemia and early reperfusion. It seemed that endogenous NO formation was observed during ischemic episodes, which is a reaction against ischemia. And the oxygen recovery after ischemic episodes can be assessed by the extent of reoxygenation and the severity of ischemia.",
keywords = "Electrochemical sensor, Myocardial ischemia-reperfusion, Nitric oxide, Oxygen, Real-time monitoring",
author = "Lee, {G. J.} and Kang, {S. W.} and Kim, {O. K.} and Kim, {S. K.} and Shin, {J. H.} and Park, {H. K.}",
year = "2012",
language = "English",
isbn = "9781466562769",
series = "Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012",
pages = "60--63",
booktitle = "Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012",
note = "Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012 ; Conference date: 18-06-2012 Through 21-06-2012",
}