Simultaneous quantification method for eleutheroside B, eleutheroside E, chiisanoside, and sesamin using reverse-phase high-performance liquid chromatography coupled with ultraviolet detection and integrated pulsed amperometric detection

We developed a method combining ultraviolet (UV) detection and integrated pulsed amperometric detection (IPAD) to simultaneously analyze eleutheroside B, eleutheroside E, chiisanoside, and sesamin. The gradient elution system allowed complete separation of all target components within 35 min, and showed limits of detection of 0.006–0.020 μg/mL and limits of quantification of 0.018–0.050 μg/mL. The linear regression coefficients of determination were 0.9990–0.9998. All inter− and intra-day precision values were below 4.89%, and the average recoveries were 97.79–104.40%. The developed approach exhibits excellent reproducibility, sensitivity, and selectivity without requiring any complicated pre-treatment, and is therefore expected to be helpful as a tool for establishing appropriate content criteria for Acanthopanax species.