TY - GEN
T1 - Slidable and valveless polymerase chain reaction-capillary electrophoresis microdevice for pathogen detection
AU - Kim, Yong Tae
AU - Choi, Jong Young
AU - Chen, Yuchao
AU - Seo, Tae Seok
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - In this experiment, we developed a novel integrated microdevice which could perform the sample loading, polymerase chain reaction (PCR), capillary electrophoresis (CE) without any microvalves or an external pump system. Microfabricated movable slide, called a slidable chamber, was switched from the sample loading to PCR amplification to CE separation. The movement of the slidable chamber was simply operated manually, and the leakage of the PCR cocktail could be minimized by the surface treatment on a chip. With a miniaturized portable genetic analyzing system, we successfully identified Staphylococcus aureus (S. aureus) on the slidable PCR-CE microdevice.
AB - In this experiment, we developed a novel integrated microdevice which could perform the sample loading, polymerase chain reaction (PCR), capillary electrophoresis (CE) without any microvalves or an external pump system. Microfabricated movable slide, called a slidable chamber, was switched from the sample loading to PCR amplification to CE separation. The movement of the slidable chamber was simply operated manually, and the leakage of the PCR cocktail could be minimized by the surface treatment on a chip. With a miniaturized portable genetic analyzing system, we successfully identified Staphylococcus aureus (S. aureus) on the slidable PCR-CE microdevice.
KW - Capillary electrophoresis
KW - Pathogen detection
KW - Polymerase chain reaction
KW - Valveless microdevice
UR - http://www.scopus.com/inward/record.url?scp=84901796275&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901796275
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1981
EP - 1983
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -