Slow-binding inhibition of soybean lipoxygenase-1 by luteolin

Tae Joung Ha; Myoung Hee Lee; Hyun Tae Kim; Hyun Sook Kwon; In Youl Baek; Isao Kubo; Dae Sik Jang

doi:10.1007/s12272-012-1014-x

Slow-binding inhibition of soybean lipoxygenase-1 by luteolin

Tae Joung Ha, Myoung Hee Lee, Hyun Tae Kim, Hyun Sook Kwon, In Youl Baek, Isao Kubo, Dae Sik Jang

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Luteolin, isolated from the seeds of Perilla frutescens (perilla seeds), inhibited the peroxidation of linoleic acid catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, Type 1) with an IC₅₀ of 5.0 μM (1.43 μg/mL) noncompetitively. The progress curves for an enzyme reaction indicate that luteolin shows slow binding kinetics. Both the initial velocity and steady-state rate in the progress curve were decreased with increasing the concentration of luteolin. The kinetic parameters, which described the inhibition by luteolin, were evaluated by nonlinear regression fits.

Original language	English
Pages (from-to)	1811-1816
Number of pages	6
Journal	Archives of Pharmacal Research
Volume	35
Issue number	10
DOIs	https://doi.org/10.1007/s12272-012-1014-x
Publication status	Published - Oct 2012

Bibliographical note

Funding Information:
This work was supported by the National Institute of Crop Science (PJ007347022011), Rural Development Administration, Korea.

Keywords

Lipoxygenase-1
Luteolin
Perilla frutescens
Slow-binding inhibitor

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10.1007/s12272-012-1014-x

Cite this

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title = "Slow-binding inhibition of soybean lipoxygenase-1 by luteolin",

abstract = "Luteolin, isolated from the seeds of Perilla frutescens (perilla seeds), inhibited the peroxidation of linoleic acid catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, Type 1) with an IC50 of 5.0 μM (1.43 μg/mL) noncompetitively. The progress curves for an enzyme reaction indicate that luteolin shows slow binding kinetics. Both the initial velocity and steady-state rate in the progress curve were decreased with increasing the concentration of luteolin. The kinetic parameters, which described the inhibition by luteolin, were evaluated by nonlinear regression fits.",

keywords = "Lipoxygenase-1, Luteolin, Perilla frutescens, Slow-binding inhibitor",

author = "Ha, {Tae Joung} and Lee, {Myoung Hee} and Kim, {Hyun Tae} and Kwon, {Hyun Sook} and Baek, {In Youl} and Isao Kubo and Jang, {Dae Sik}",

note = "Funding Information: This work was supported by the National Institute of Crop Science (PJ007347022011), Rural Development Administration, Korea.",

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month = oct,

doi = "10.1007/s12272-012-1014-x",

language = "English",

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T1 - Slow-binding inhibition of soybean lipoxygenase-1 by luteolin

AU - Ha, Tae Joung

AU - Lee, Myoung Hee

AU - Kim, Hyun Tae

AU - Kwon, Hyun Sook

AU - Baek, In Youl

AU - Kubo, Isao

AU - Jang, Dae Sik

N1 - Funding Information: This work was supported by the National Institute of Crop Science (PJ007347022011), Rural Development Administration, Korea.

PY - 2012/10

Y1 - 2012/10

N2 - Luteolin, isolated from the seeds of Perilla frutescens (perilla seeds), inhibited the peroxidation of linoleic acid catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, Type 1) with an IC50 of 5.0 μM (1.43 μg/mL) noncompetitively. The progress curves for an enzyme reaction indicate that luteolin shows slow binding kinetics. Both the initial velocity and steady-state rate in the progress curve were decreased with increasing the concentration of luteolin. The kinetic parameters, which described the inhibition by luteolin, were evaluated by nonlinear regression fits.

AB - Luteolin, isolated from the seeds of Perilla frutescens (perilla seeds), inhibited the peroxidation of linoleic acid catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, Type 1) with an IC50 of 5.0 μM (1.43 μg/mL) noncompetitively. The progress curves for an enzyme reaction indicate that luteolin shows slow binding kinetics. Both the initial velocity and steady-state rate in the progress curve were decreased with increasing the concentration of luteolin. The kinetic parameters, which described the inhibition by luteolin, were evaluated by nonlinear regression fits.

KW - Lipoxygenase-1

KW - Luteolin

KW - Perilla frutescens

KW - Slow-binding inhibitor

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U2 - 10.1007/s12272-012-1014-x

DO - 10.1007/s12272-012-1014-x

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AN - SCOPUS:84872103749

SN - 0253-6269

VL - 35

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EP - 1816

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

IS - 10

ER -

Slow-binding inhibition of soybean lipoxygenase-1 by luteolin

Abstract

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Keywords

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