Sojucktang induces apoptosis via loss of mitochondrial membrane potential and caspase-3 activation in KLE human endometrial cancer cells

Yeon Suk Oh; Hee Young Kwon; Soo Jin Jeong; Ki Young Park; Sun Young Kim; Hyo Jung Lee; Hyo Jeong Lee; Eun Ok Lee; Kwang Seok Ahn; Sung Hoon Kim

doi:10.1007/s11434-009-0656-7

Sojucktang induces apoptosis via loss of mitochondrial membrane potential and caspase-3 activation in KLE human endometrial cancer cells

Yeon Suk Oh
, Hee Young Kwon
, Soo Jin Jeong
, Ki Young Park
, Sun Young Kim
, Hyo Jung Lee
, Hyo Jeong Lee
, Eun Ok Lee
, Kwang Seok Ahn
, Sung Hoon Kim

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Sojucktang (SJT) has long been used for the treatment of endometrial diseases in Korea. However, the mechanisms responsible for the SJT-induced apoptosis in endometrial cancer cells remain unclear. In the present study, SJT was demonstrated to show cytotoxic effect and induce apoptotic cell death via mitochondrial regulation in KLE endometrial cancer cells. Linderae Radix, Glycyrrhizae Radix, Zedoariae Rhizoma, Trogopterorum Faeces and Agelicae Gigantis Radix were found to be the potent constituent herbs of SJT to significantly decrease the viability of KLE cells by a tetra zolium salt (XTT) assay. Apoptotic bodies were observed in SJT-treated KLE cells by 4′-6-diamidino-2-phenylindole (DAPI) and TdT-mediated-dUTP nick-end labeling (TUNEL) assay. SJT also increased sub-G1 DNA contents of the cell cycle undergoing apoptosis in a dose-dependent manner. Furthermore, it was observed that SJT activated caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP), and decreased mitochondrial membrane potential in a dose-dependent manner. Taken together, this study shows that SJT exerts anti-tumor activity against KLE endometrial cancer cells via mitochondrial dependent apoptosis induction.

Original language	English
Pages (from-to)	4387-4392
Number of pages	6
Journal	Chinese Science Bulletin
Volume	54
Issue number	23
DOIs	https://doi.org/10.1007/s11434-009-0656-7
Publication status	Published - Dec 2009

Bibliographical note

Funding Information:
Received June 15, 2009; accepted August 10, 2009 doi: 10.1007/s11434-009-0656-7 †Corresponding author (email: [email protected]) Supported by the Korea Science and Engineering Foundation (Grant No. KOSEF) funded by the Korea government (MEST) (Grant No. R13-2007-019-00000-0)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
Caspase-3
KLE cells
Mitochondria
Sojucktang

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10.1007/s11434-009-0656-7

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@article{e97a82c6d6e54c8ea79b521b0dcbb39c,

title = "Sojucktang induces apoptosis via loss of mitochondrial membrane potential and caspase-3 activation in KLE human endometrial cancer cells",

abstract = "Sojucktang (SJT) has long been used for the treatment of endometrial diseases in Korea. However, the mechanisms responsible for the SJT-induced apoptosis in endometrial cancer cells remain unclear. In the present study, SJT was demonstrated to show cytotoxic effect and induce apoptotic cell death via mitochondrial regulation in KLE endometrial cancer cells. Linderae Radix, Glycyrrhizae Radix, Zedoariae Rhizoma, Trogopterorum Faeces and Agelicae Gigantis Radix were found to be the potent constituent herbs of SJT to significantly decrease the viability of KLE cells by a tetra zolium salt (XTT) assay. Apoptotic bodies were observed in SJT-treated KLE cells by 4′-6-diamidino-2-phenylindole (DAPI) and TdT-mediated-dUTP nick-end labeling (TUNEL) assay. SJT also increased sub-G1 DNA contents of the cell cycle undergoing apoptosis in a dose-dependent manner. Furthermore, it was observed that SJT activated caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP), and decreased mitochondrial membrane potential in a dose-dependent manner. Taken together, this study shows that SJT exerts anti-tumor activity against KLE endometrial cancer cells via mitochondrial dependent apoptosis induction.",

keywords = "Apoptosis, Caspase-3, KLE cells, Mitochondria, Sojucktang",

author = "Oh, \{Yeon Suk\} and Kwon, \{Hee Young\} and Jeong, \{Soo Jin\} and Park, \{Ki Young\} and Kim, \{Sun Young\} and Lee, \{Hyo Jung\} and Lee, \{Hyo Jeong\} and Lee, \{Eun Ok\} and Ahn, \{Kwang Seok\} and Kim, \{Sung Hoon\}",

note = "Funding Information: Received June 15, 2009; accepted August 10, 2009 doi: 10.1007/s11434-009-0656-7 †Corresponding author (email: [email protected]) Supported by the Korea Science and Engineering Foundation (Grant No. KOSEF) funded by the Korea government (MEST) (Grant No. R13-2007-019-00000-0)",

year = "2009",

month = dec,

doi = "10.1007/s11434-009-0656-7",

language = "English",

volume = "54",

pages = "4387--4392",

journal = "Chinese Science Bulletin",

issn = "1001-6538",

publisher = "Science Press",

number = "23",

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TY - JOUR

T1 - Sojucktang induces apoptosis via loss of mitochondrial membrane potential and caspase-3 activation in KLE human endometrial cancer cells

AU - Oh, Yeon Suk

AU - Kwon, Hee Young

AU - Jeong, Soo Jin

AU - Park, Ki Young

AU - Kim, Sun Young

AU - Lee, Hyo Jung

AU - Lee, Hyo Jeong

AU - Lee, Eun Ok

AU - Ahn, Kwang Seok

AU - Kim, Sung Hoon

N1 - Funding Information: Received June 15, 2009; accepted August 10, 2009 doi: 10.1007/s11434-009-0656-7 †Corresponding author (email: [email protected]) Supported by the Korea Science and Engineering Foundation (Grant No. KOSEF) funded by the Korea government (MEST) (Grant No. R13-2007-019-00000-0)

PY - 2009/12

Y1 - 2009/12

N2 - Sojucktang (SJT) has long been used for the treatment of endometrial diseases in Korea. However, the mechanisms responsible for the SJT-induced apoptosis in endometrial cancer cells remain unclear. In the present study, SJT was demonstrated to show cytotoxic effect and induce apoptotic cell death via mitochondrial regulation in KLE endometrial cancer cells. Linderae Radix, Glycyrrhizae Radix, Zedoariae Rhizoma, Trogopterorum Faeces and Agelicae Gigantis Radix were found to be the potent constituent herbs of SJT to significantly decrease the viability of KLE cells by a tetra zolium salt (XTT) assay. Apoptotic bodies were observed in SJT-treated KLE cells by 4′-6-diamidino-2-phenylindole (DAPI) and TdT-mediated-dUTP nick-end labeling (TUNEL) assay. SJT also increased sub-G1 DNA contents of the cell cycle undergoing apoptosis in a dose-dependent manner. Furthermore, it was observed that SJT activated caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP), and decreased mitochondrial membrane potential in a dose-dependent manner. Taken together, this study shows that SJT exerts anti-tumor activity against KLE endometrial cancer cells via mitochondrial dependent apoptosis induction.

AB - Sojucktang (SJT) has long been used for the treatment of endometrial diseases in Korea. However, the mechanisms responsible for the SJT-induced apoptosis in endometrial cancer cells remain unclear. In the present study, SJT was demonstrated to show cytotoxic effect and induce apoptotic cell death via mitochondrial regulation in KLE endometrial cancer cells. Linderae Radix, Glycyrrhizae Radix, Zedoariae Rhizoma, Trogopterorum Faeces and Agelicae Gigantis Radix were found to be the potent constituent herbs of SJT to significantly decrease the viability of KLE cells by a tetra zolium salt (XTT) assay. Apoptotic bodies were observed in SJT-treated KLE cells by 4′-6-diamidino-2-phenylindole (DAPI) and TdT-mediated-dUTP nick-end labeling (TUNEL) assay. SJT also increased sub-G1 DNA contents of the cell cycle undergoing apoptosis in a dose-dependent manner. Furthermore, it was observed that SJT activated caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP), and decreased mitochondrial membrane potential in a dose-dependent manner. Taken together, this study shows that SJT exerts anti-tumor activity against KLE endometrial cancer cells via mitochondrial dependent apoptosis induction.

KW - Apoptosis

KW - Caspase-3

KW - KLE cells

KW - Mitochondria

KW - Sojucktang

UR - https://www.scopus.com/pages/publications/74749091262

U2 - 10.1007/s11434-009-0656-7

DO - 10.1007/s11434-009-0656-7

M3 - Article

AN - SCOPUS:74749091262

SN - 1001-6538

VL - 54

SP - 4387

EP - 4392

JO - Chinese Science Bulletin

JF - Chinese Science Bulletin

IS - 23

ER -

Sojucktang induces apoptosis via loss of mitochondrial membrane potential and caspase-3 activation in KLE human endometrial cancer cells

Abstract

Bibliographical note

UN SDGs

Keywords

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