Solid-phase colorimetric method for the quantification of fucoidan

Jung Min Lee; Z. U. Shin; Gafurjon T. Mavlonov; Ibrokhim Y. Abdurakhmonov; Tae Hoo Yi

doi:10.1007/s12010-012-9837-y

Solid-phase colorimetric method for the quantification of fucoidan

Jung Min Lee
, Z. U. Shin
, Gafurjon T. Mavlonov
, Ibrokhim Y. Abdurakhmonov
, Tae Hoo Yi

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

We described the simple, selective, and rapid method for determination of fucoidans using methylene blue staining of sulfated polysaccharides, immobilized into filter paper and consequent optic density (at A663nm) measurement of the eluted dye from filter paper. This solid-phase method allows selective determination of 1-20 μg fucoidan in presence of potentially interfering compounds (alginic acid, DNA, salts, proteins, and detergents). Further, we demonstrated the alternative way of using image processing software for fucoidan quantification without extraction of methylene blue dye from stained spots of fucoidan-dye complex.

Original language	English
Pages (from-to)	1019-1024
Number of pages	6
Journal	Applied Biochemistry and Biotechnology
Volume	168
Issue number	5
DOIs	https://doi.org/10.1007/s12010-012-9837-y
Publication status	Published - Nov 2012

Bibliographical note

Funding Information:
Acknowledgments This research was supported by Fishery Commercialization Support Program Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea, grant no 109203-03-3-CG000.

Keywords

Fucoidan
Methylene blue binding
Quantity one® soft
Solid-phase colorimetry
Spot quantitation
Sulfated polysaccharides

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10.1007/s12010-012-9837-y

Cite this

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title = "Solid-phase colorimetric method for the quantification of fucoidan",

abstract = "We described the simple, selective, and rapid method for determination of fucoidans using methylene blue staining of sulfated polysaccharides, immobilized into filter paper and consequent optic density (at A663nm) measurement of the eluted dye from filter paper. This solid-phase method allows selective determination of 1-20 μg fucoidan in presence of potentially interfering compounds (alginic acid, DNA, salts, proteins, and detergents). Further, we demonstrated the alternative way of using image processing software for fucoidan quantification without extraction of methylene blue dye from stained spots of fucoidan-dye complex.",

keywords = "Fucoidan, Methylene blue binding, Quantity one{\textregistered} soft, Solid-phase colorimetry, Spot quantitation, Sulfated polysaccharides",

author = "Lee, \{Jung Min\} and Shin, \{Z. U.\} and Mavlonov, \{Gafurjon T.\} and Abdurakhmonov, \{Ibrokhim Y.\} and Yi, \{Tae Hoo\}",

note = "Funding Information: Acknowledgments This research was supported by Fishery Commercialization Support Program Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea, grant no 109203-03-3-CG000.",

year = "2012",

month = nov,

doi = "10.1007/s12010-012-9837-y",

language = "English",

volume = "168",

pages = "1019--1024",

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T1 - Solid-phase colorimetric method for the quantification of fucoidan

AU - Lee, Jung Min

AU - Shin, Z. U.

AU - Mavlonov, Gafurjon T.

AU - Abdurakhmonov, Ibrokhim Y.

AU - Yi, Tae Hoo

N1 - Funding Information: Acknowledgments This research was supported by Fishery Commercialization Support Program Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea, grant no 109203-03-3-CG000.

PY - 2012/11

Y1 - 2012/11

N2 - We described the simple, selective, and rapid method for determination of fucoidans using methylene blue staining of sulfated polysaccharides, immobilized into filter paper and consequent optic density (at A663nm) measurement of the eluted dye from filter paper. This solid-phase method allows selective determination of 1-20 μg fucoidan in presence of potentially interfering compounds (alginic acid, DNA, salts, proteins, and detergents). Further, we demonstrated the alternative way of using image processing software for fucoidan quantification without extraction of methylene blue dye from stained spots of fucoidan-dye complex.

AB - We described the simple, selective, and rapid method for determination of fucoidans using methylene blue staining of sulfated polysaccharides, immobilized into filter paper and consequent optic density (at A663nm) measurement of the eluted dye from filter paper. This solid-phase method allows selective determination of 1-20 μg fucoidan in presence of potentially interfering compounds (alginic acid, DNA, salts, proteins, and detergents). Further, we demonstrated the alternative way of using image processing software for fucoidan quantification without extraction of methylene blue dye from stained spots of fucoidan-dye complex.

KW - Fucoidan

KW - Methylene blue binding

KW - Quantity one® soft

KW - Solid-phase colorimetry

KW - Spot quantitation

KW - Sulfated polysaccharides

UR - https://www.scopus.com/pages/publications/84871920380

U2 - 10.1007/s12010-012-9837-y

DO - 10.1007/s12010-012-9837-y

M3 - Article

C2 - 22903325

AN - SCOPUS:84871920380

SN - 0273-2289

VL - 168

SP - 1019

EP - 1024

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 5

ER -

Solid-phase colorimetric method for the quantification of fucoidan

Abstract

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Keywords

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