Spinning of a gigantic bundle of hollow fibrils by a spirally moving higher plant protoplast

Tomoko Seyama; Satoshi Kimura; Hamako Sasamoto; Hisashi Abe; Tetsuo Kondo

doi:10.1007/s00425-008-0689-1

Spinning of a gigantic bundle of hollow fibrils by a spirally moving higher plant protoplast

Tomoko Seyama
, Satoshi Kimura
, Hamako Sasamoto
, Hisashi Abe
, Tetsuo Kondo

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A unique fiber spinning was found in protoplasts from white birch (Betula platyphylla) leaves under an acidic medium containing high concentration of Ca²⁺. After expanding from 10 to 100 μm in diameter under the culture condition, the protoplast started secreting a gigantic fiber while moving in a spiral way. Real time video analyses elucidated that the orientation, rate and pattern of the motion were directed due to the inverse force of the fiber spinning. Moreover, observation using several microscopic methods accompanied with histochemical staining and nuclear magnetic resonance (NMR) analysis indicated that the fiber was composed of 400-500 nm wide (1→3)-β-glucan hollow sub-fibrils. This entire phenomenon may be a response against the stress imposed. The observation presented provides an understanding of the unique relationship between fiber spinning and the bottom-up fiber fabrication from nano to micro scales.

Original language	English
Pages (from-to)	1187-1197
Number of pages	11
Journal	Planta
Volume	227
Issue number	6
DOIs	https://doi.org/10.1007/s00425-008-0689-1
Publication status	Published - May 2008

Bibliographical note

Funding Information:
Acknowledgments This research was supported partly by a Grant-in-Aid for Scientific Research (No. 14360101), Japan Society for the Promotion of Science (JSPS). The authors are grateful to Dr. Adya P. Singh at ENSIS (The Joint Forces of CSIRO & SION) for the editing of the text, and to Dr. Kazuaki Shikii for the NMR measurement (Nissan Chemical Industries, Ltd.). SEKI TECHNOTRON CORP. is also acknowledged by the assistance for observation using sectioning microscopy.

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

Calcium ions
Callose
Fiber spinning
Protoplast
Spiral movement

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10.1007/s00425-008-0689-1

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title = "Spinning of a gigantic bundle of hollow fibrils by a spirally moving higher plant protoplast",

abstract = "A unique fiber spinning was found in protoplasts from white birch (Betula platyphylla) leaves under an acidic medium containing high concentration of Ca2+. After expanding from 10 to 100 μm in diameter under the culture condition, the protoplast started secreting a gigantic fiber while moving in a spiral way. Real time video analyses elucidated that the orientation, rate and pattern of the motion were directed due to the inverse force of the fiber spinning. Moreover, observation using several microscopic methods accompanied with histochemical staining and nuclear magnetic resonance (NMR) analysis indicated that the fiber was composed of 400-500 nm wide (1→3)-β-glucan hollow sub-fibrils. This entire phenomenon may be a response against the stress imposed. The observation presented provides an understanding of the unique relationship between fiber spinning and the bottom-up fiber fabrication from nano to micro scales.",

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author = "Tomoko Seyama and Satoshi Kimura and Hamako Sasamoto and Hisashi Abe and Tetsuo Kondo",

note = "Funding Information: Acknowledgments This research was supported partly by a Grant-in-Aid for Scientific Research (No. 14360101), Japan Society for the Promotion of Science (JSPS). The authors are grateful to Dr. Adya P. Singh at ENSIS (The Joint Forces of CSIRO \& SION) for the editing of the text, and to Dr. Kazuaki Shikii for the NMR measurement (Nissan Chemical Industries, Ltd.). SEKI TECHNOTRON CORP. is also acknowledged by the assistance for observation using sectioning microscopy. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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doi = "10.1007/s00425-008-0689-1",

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AU - Sasamoto, Hamako

AU - Abe, Hisashi

AU - Kondo, Tetsuo

N1 - Funding Information: Acknowledgments This research was supported partly by a Grant-in-Aid for Scientific Research (No. 14360101), Japan Society for the Promotion of Science (JSPS). The authors are grateful to Dr. Adya P. Singh at ENSIS (The Joint Forces of CSIRO & SION) for the editing of the text, and to Dr. Kazuaki Shikii for the NMR measurement (Nissan Chemical Industries, Ltd.). SEKI TECHNOTRON CORP. is also acknowledged by the assistance for observation using sectioning microscopy. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

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N2 - A unique fiber spinning was found in protoplasts from white birch (Betula platyphylla) leaves under an acidic medium containing high concentration of Ca2+. After expanding from 10 to 100 μm in diameter under the culture condition, the protoplast started secreting a gigantic fiber while moving in a spiral way. Real time video analyses elucidated that the orientation, rate and pattern of the motion were directed due to the inverse force of the fiber spinning. Moreover, observation using several microscopic methods accompanied with histochemical staining and nuclear magnetic resonance (NMR) analysis indicated that the fiber was composed of 400-500 nm wide (1→3)-β-glucan hollow sub-fibrils. This entire phenomenon may be a response against the stress imposed. The observation presented provides an understanding of the unique relationship between fiber spinning and the bottom-up fiber fabrication from nano to micro scales.

AB - A unique fiber spinning was found in protoplasts from white birch (Betula platyphylla) leaves under an acidic medium containing high concentration of Ca2+. After expanding from 10 to 100 μm in diameter under the culture condition, the protoplast started secreting a gigantic fiber while moving in a spiral way. Real time video analyses elucidated that the orientation, rate and pattern of the motion were directed due to the inverse force of the fiber spinning. Moreover, observation using several microscopic methods accompanied with histochemical staining and nuclear magnetic resonance (NMR) analysis indicated that the fiber was composed of 400-500 nm wide (1→3)-β-glucan hollow sub-fibrils. This entire phenomenon may be a response against the stress imposed. The observation presented provides an understanding of the unique relationship between fiber spinning and the bottom-up fiber fabrication from nano to micro scales.

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KW - Protoplast

KW - Spiral movement

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JO - Planta

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IS - 6

ER -

Spinning of a gigantic bundle of hollow fibrils by a spirally moving higher plant protoplast

Abstract

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Keywords

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