TY - JOUR
T1 - Structural identification of polyhydroxyalkanoic acid (PHA) containing 4-hydroxyalkanoic acids by gas chromatography-mass spectrometry (GC-MS) and its application to bacteria screening
AU - Lee, Eun Yeol
AU - Choi, Cha Yong
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1997
Y1 - 1997
N2 - The methanolysis products of polyhydroxyalkanoic acids (PHAs) containing 4-hydroxybutyric acid (4HB), 4-hydroxyvaleric acid (4HV), and 4-hydroxyhexanoic acid (4HHx), when analyzed by GC-MS, showed two major chromatographic peaks with characteristic retention times of each methyl ester of 4-hydroxyalkanoic acid and the corresponding γ-lactone (γ-butyrolactone, γ-valerolactone, γ-caprolaetone, respectively). The method and results of GC-MS could be incorporated into an efficient screening procedure for isolation of bacterial strains which could accumulate a PHA containing 4-hydroxyalkanoic acid as the principal monomer from structurally related carbon substrates.
AB - The methanolysis products of polyhydroxyalkanoic acids (PHAs) containing 4-hydroxybutyric acid (4HB), 4-hydroxyvaleric acid (4HV), and 4-hydroxyhexanoic acid (4HHx), when analyzed by GC-MS, showed two major chromatographic peaks with characteristic retention times of each methyl ester of 4-hydroxyalkanoic acid and the corresponding γ-lactone (γ-butyrolactone, γ-valerolactone, γ-caprolaetone, respectively). The method and results of GC-MS could be incorporated into an efficient screening procedure for isolation of bacterial strains which could accumulate a PHA containing 4-hydroxyalkanoic acid as the principal monomer from structurally related carbon substrates.
UR - https://www.scopus.com/pages/publications/0030999871
U2 - 10.1023/a:1018401513621
DO - 10.1023/a:1018401513621
M3 - Article
AN - SCOPUS:0030999871
SN - 0951-208X
VL - 11
SP - 167
EP - 171
JO - Biotechnology Techniques
JF - Biotechnology Techniques
IS - 3
ER -