Substance P enhances cellular migration and inhibits senescence in human dermal fibroblasts under hyperglycemic conditions

Jinyeong Yu, Donghyun Nam, Ki Sook Park

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Diabetes induces cellular dysfunction in dermal fibroblasts, such as impairment in migration, which is a major cause of chronic wound. Here, we demonstrated that the migration of human dermal fibroblasts was impaired under a high glucose culture condition. Substance P (SP) rescued the impaired migration of the fibroblasts. The activity of Rac1, Rho-associated kinase (ROCK), and Src was required for SP-mediated rescue of fibroblast migration. SP activated Rac1 and Src, whereas, NSC23766, a Rac1 inhibitor, and PP1 and PP2, Src inhibitors, inhibited SP-mediated enhancement of fibroblast migration. Y-27632, a ROCK inhibitor, inhibited the SP-mediated rescue of fibroblast migration. Senescence-associated β-galactosidase activity increased in human dermal fibroblasts cultured in a high glucose environment, but SP inhibited the β-galactosidase activity of the fibroblasts. These results suggest that SP promotes the migration of human dermal fibroblasts in diabetic-condition-mimicking cultures via the activity of Rac1, ROCK, and Src, and inhibits fibroblast senescence in hyperglycemic cultures.

Original languageEnglish
Pages (from-to)917-923
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume522
Issue number4
DOIs
Publication statusPublished - 19 Feb 2020

Keywords

  • Fibroblast
  • Migration
  • Senescence
  • Substance P

Fingerprint

Dive into the research topics of 'Substance P enhances cellular migration and inhibits senescence in human dermal fibroblasts under hyperglycemic conditions'. Together they form a unique fingerprint.

Cite this