Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay

Seungah Lee; Junghwa Lee; Seong Ho Kang

doi:10.1039/d2an01734b

Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay

Seungah Lee
, Junghwa Lee
, Seong Ho Kang

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We develop a supersensitive “turn-on format” fluorescence sandwich immunoassay for detecting small single molecules. Gold nanoplate-based biotin antibodies and streptavidin-fluorophores were used instead of streptavidin-horseradish peroxidase reacting with a biotin tracer in a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). Our platform showed a low detection limit of 5 zeptomolar (5 × 10^-21 M), 5.4 × 10¹⁰ times higher detection sensitivity than the conventional tune-off format ELISA.

Original language	English
Pages (from-to)	714-718
Number of pages	5
Journal	Analyst
Volume	148
Issue number	4
DOIs	https://doi.org/10.1039/d2an01734b
Publication status	Published - 20 Jan 2023

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© 2023 The Royal Society of Chemistry.

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10.1039/d2an01734b

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abstract = "We develop a supersensitive “turn-on format” fluorescence sandwich immunoassay for detecting small single molecules. Gold nanoplate-based biotin antibodies and streptavidin-fluorophores were used instead of streptavidin-horseradish peroxidase reacting with a biotin tracer in a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). Our platform showed a low detection limit of 5 zeptomolar (5 × 10-21 M), 5.4 × 1010 times higher detection sensitivity than the conventional tune-off format ELISA.",

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AU - Lee, Seungah

AU - Lee, Junghwa

AU - Kang, Seong Ho

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Y1 - 2023/1/20

N2 - We develop a supersensitive “turn-on format” fluorescence sandwich immunoassay for detecting small single molecules. Gold nanoplate-based biotin antibodies and streptavidin-fluorophores were used instead of streptavidin-horseradish peroxidase reacting with a biotin tracer in a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). Our platform showed a low detection limit of 5 zeptomolar (5 × 10-21 M), 5.4 × 1010 times higher detection sensitivity than the conventional tune-off format ELISA.

AB - We develop a supersensitive “turn-on format” fluorescence sandwich immunoassay for detecting small single molecules. Gold nanoplate-based biotin antibodies and streptavidin-fluorophores were used instead of streptavidin-horseradish peroxidase reacting with a biotin tracer in a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). Our platform showed a low detection limit of 5 zeptomolar (5 × 10-21 M), 5.4 × 1010 times higher detection sensitivity than the conventional tune-off format ELISA.

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U2 - 10.1039/d2an01734b

DO - 10.1039/d2an01734b

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Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay

Abstract

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